Efficient cell delivery mediated by lipid‐specific endosomal escape of supercharged branched peptides

• Published in: Traffic (Copenhagen, Denmark) Vol. 19; no. 6; pp. 421 - 435
• Main Authors: Brock, Dakota J., Kustigian, Lauren, Jiang, Mengqiu, Graham, Kristin, Wang, Ting‐Yi, Erazo‐Oliveras, Alfredo, Najjar, Kristina, Zhang, Junjie, Rye, Hays, Pellois, Jean‐Philippe
• Format: Journal Article
• Language: English
• Published: Former Munksgaard John Wiley & Sons A/S 01.06.2018; Wiley Subscription Services, Inc
• Subjects: bis(monoacylglycero)phosphate; Bone morphogenetic proteins; Cell Line, Tumor; cellular delivery; cell‐penetrating peptides; Cytosol - metabolism; endosomal escape; Endosomes - metabolism; Flocculation; HeLa Cells; HIV; Human immunodeficiency virus; Humans; Intracellular Membranes - metabolism; Leakage; Lipid bilayers; Lipid Bilayers - metabolism; Lipids; Lipids - chemistry; Liposomes - metabolism; Macromolecules; membrane leakage; Peptides - metabolism; supercharged molecules; Tat protein; cellular delivery; bis(monoacylglycero)phosphate; membrane leakage; endosomal escape; supercharged molecules; cell-penetrating peptides

Various densely charged polycationic species, whether of biological or synthetic origin, can penetrate human cells, albeit with variable efficiencies. The molecular underpinnings involved in such transport remain unclear. Herein, we assemble 1, 2 or 3 copies of the HIV peptide TAT on a synthetic sca...