Borreliacidal activity of saliva of the tick Amblyomma americanum

• Published in: Medical and veterinary entomology Vol. 19; no. 1; pp. 90 - 95
• Main Authors: Ledin, K.E, Zeidner, N.S, Ribeiro, J.M.C, Biggerstaff, B.J, Dolan, M.C, Dietrich, G, Vredevoe, L, Piesman, J
• Format: Journal Article
• Language: English
• Published: Oxford, UK; Malden, USA Blackwell Science Ltd 01.03.2005
• Subjects: Amblyomma americanum; Animals; antibacterial properties; Arachnid Vectors - chemistry; Arachnid Vectors - physiology; Borrelia burgdorferi; Borrelia burgdorferi - physiology; borreliacidal effect; cytotoxicity; disease vectors; duration of exposure; Female; Ixodes scapularis; Ixodidae; Ixodidae - chemistry; Ixodidae - physiology; Lyme disease; pathogen survival; Pilocarpine - analysis; Rabbits; refractory vectors; saliva; Saliva - chemistry; Saliva - physiology; salivary glands; salivation; Species Specificity; tick saliva; ticks; vector competence; USA
• ISSN: 0269-283X; 1365-2915
• DOI: 10.1111/j.0269-283X.2005.00546.x

Amblyomma americanum (Linneaus) (Acari: Ixodidae), an important tick vector of human and animal disease, is not a competent vector of the bacterial agent of Lyme disease, Borrelia burgdorferi, although its range overlaps the geographical distribution of Lyme disease within the United States. A possible mechanism that could prevent acquisition of B. burgdorferi spirochetes from infected hosts is the toxic effect of A. americanum saliva on B. burgdorferi. The data presented here indicate that after 24 and 48 h of exposure to A. americanum saliva, significantly fewer B. burgdorferi were alive compared to treatment controls as assessed by spirochete motility under dark-field microscopy and resistance to the dead stain, propidium iodide. After 48 h, fewer than 13% of saliva-exposed B. burgdorferi were alive. In contrast, significantly more B. burgdorferi exposed to Ixodes scapularis (Acari: Ixodidae) saliva survived after 24 or 48 h compared to A. americanum saliva or treatment controls.