Bacteriological profile of neonatal sepsis and antibiotic susceptibility pattern of isolates admitted at Kanti Children's Hospital, Kathmandu, Nepal

• Published in: BMC research notes Vol. 11; no. 1; p. 301
• Main Authors: Yadav, Nikita Singh, Sharma, Saroj, Chaudhary, Dhiraj Kumar, Panthi, Prabhat, Pokhrel, Pankaj, Shrestha, Anil, Mandal, Pappu Kumar
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 15.05.2018; BioMed Central; BMC
• Subjects: Age; Ampicillin; Anti-Bacterial Agents - pharmacology; Antibiotic susceptibility; Antibiotics; Bacteria; Bacterial infections; Bacteriological profile; Birth weight; Births; Blood culture; Care and treatment; Cefotaxime; Complications and side effects; Developing countries; Dosage and administration; Drug resistance; Drug Resistance, Bacterial; E coli; Female; Gentamicin; Gestational age; Gram-Negative Bacterial Infections - blood; Gram-Negative Bacterial Infections - epidemiology; Gram-Negative Bacterial Infections - microbiology; Gram-positive bacteria; Gram-Positive Bacterial Infections - blood; Gram-Positive Bacterial Infections - epidemiology; Gram-Positive Bacterial Infections - microbiology; Hospitals; Humans; Imipenem; Infant mortality; Infant, Newborn; Klebsiella - drug effects; Klebsiella - isolation & purification; Laboratories; LDCs; Low birth weight; Male; Meropenem; Microbial drug resistance; Microbial Sensitivity Tests; Morbidity; Neonatal sepsis; Neonatal Sepsis - blood; Neonatal Sepsis - epidemiology; Neonatal Sepsis - microbiology; Neonates; Nepal; Nepal - epidemiology; Patient outcomes; Quality management; Research Note; Risk factors; Salmonella; Sepsis; Septicemia; Staphylococcus aureus - drug effects; Staphylococcus aureus - isolation & purification; Staphylococcus infections; Streptococcus infections; Nepal; Neonates; Bacteriological profile; Antibiotic susceptibility; Neonatal sepsis
• ISSN: 1756-0500; 1756-0500
• DOI: 10.1186/s13104-018-3394-6

Neonatal sepsis is a major cause of morbidity and mortality of newborns (< 1 month of age). Septicemia and drug resistance is a predominant issue for neonatal death in Nepal. This study is intended to find bacteriological profile of neonatal sepsis and antibiotic susceptibility pattern of the isolates from neonates at Kanti Children's Hospital, Kathmandu, Nepal. Out of 350 suspected cases of neonatal sepsis, 59 (16.9%) cases showed positive blood culture. The prevalent of positive blood culture with different neonatal risk factors (sex, age, birth weight, gestational age, and delivery mode) showed highest positive bacterial growth in male (52.3%); 3 or above 3 days age (71.2%); low birth weight (62.7%); preterm gestational age (31.4%); and caesarean delivery mode (63.3%). Among positive cases, the bacteriological profile was found highest for Staphylococcus aureus (35.6%) followed by Klebsiella pneumoniae (15.3%). The most sensitive and resistive antibiotics among Gram-positive isolates were gentamicin (93%) and ampicillin (78%), respectively. Meropenem and imipenem showed highest 100% effective and cefotaxime was least (28%) sensitive among Gram-negative isolates. This concludes broad ranges of bacteria are associated with neonatal sepsis and revealed variation in antibiotic susceptibility pattern among bacterial isolates.