Toll‐Like Receptor 9 Deficiency Breaks Tolerance to RNA‐Associated Antigens and Up‐Regulates Toll‐Like Receptor 7 Protein in Sle1 Mice

• Published in: Arthritis & rheumatology (Hoboken, N.J.) Vol. 70; no. 10; pp. 1597 - 1609
• Main Authors: Celhar, Teja, Yasuga, Hiroko, Lee, Hui Yin, Zharkova, Olga, Tripathi, Shubhita, Thornhill, Susannah I., Lu, Hao K., Au, Bijin, Lim, Lina H. K., Thamboo, Thomas P., Akira, Shizuo, Wakeland, Edward K., Connolly, John E., Fairhurst, Anna‐Marie
• Format: Journal Article
• Language: English
• Published: United States Wiley Subscription Services, Inc 01.10.2018; John Wiley and Sons Inc
• Subjects: Ablation; Animal models; Animals; Antibodies; Antigen presentation; Antigen-antibody complexes; Antigens; Antigens - immunology; Autoantibodies; Autoimmune diseases; Autoimmunity; Chronic conditions; Dendritic cells; Dependence; Disease Models, Animal; Glomerulonephritis; Immune system; Immunoglobulins; Immunological tolerance; Infiltration; Kidneys; Lupus; Lupus Erythematosus, Systemic - immunology; Lupus Nephritis - immunology; Lymphocytes B; Mice; Original; Proteins; Receptors; Ribonucleic acid; RNA; RNA - immunology; Splenomegaly; Systemic Lupus Erythematosus; Toll-Like Receptor 7 - immunology; Toll-Like Receptor 9 - deficiency; Toll-Like Receptor 9 - immunology; Toll-like receptors; Up-Regulation - immunology
• ISSN: 2326-5191; 2326-5205
• DOI: 10.1002/art.40535

Objective Toll‐like receptors (TLRs) 7 and 9 are important innate signaling molecules with opposing roles in the development and progression of systemic lupus erythematosus (SLE). While multiple studies support the notion of a dependency on TLR‐7 for disease development, genetic ablation of TLR‐9 results in severe disease with glomerulonephritis (GN) by a largely unknown mechanism. This study was undertaken to examine the suppressive role of TLR‐9 in the development of severe lupus in a mouse model. Methods We crossed Sle1 lupus‐prone mice with TLR‐9–deficient mice to generate Sle1TLR‐9−/− mice. Mice ages 4.5–6.5 months were evaluated for severe autoimmunity by assessing splenomegaly, GN, immune cell populations, autoantibody and total Ig profiles, kidney dendritic cell (DC) function, and TLR‐7 protein expression. Mice ages 8–10 weeks were used for functional B cell studies, Ig profiling, and determination of TLR‐7 expression. Results Sle1TLR‐9−/− mice developed severe disease similar to TLR‐9–deficient MRL and Nba2 models. Sle1TLR‐9−/− mouse B cells produced more class‐switched antibodies, and the autoantibody repertoire was skewed toward RNA‐containing antigens. GN in these mice was associated with DC infiltration, and purified Sle1TLR‐9−/− mouse renal DCs were more efficient at TLR‐7–dependent antigen presentation and expressed higher levels of TLR‐7 protein. Importantly, this increase in TLR‐7 expression occurred prior to disease development, indicating a role in the initiation stages of tissue destruction. Conclusion The increase in TLR‐7–reactive immune complexes, and the concomitant enhanced expression of their receptor, promotes inflammation and disease in Sle1TLR9−/− mice.