Efficient production of chicken egg yolk antibodies against a conserved mammalian protein

The egg yolk of immunized chicken is a rich and inexpensive source of specific polyclonal antibodies. In this paper we show that 20-30 micrograms of a highly conserved mammalian protein, as exemplified by proliferating cell nuclear antigen, are sufficient to induce an immune response. Immunoblot ana...

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Bibliographic Details
Published inThe FASEB journal Vol. 4; no. 8; p. 2528
Main Authors Gassmann, M, Thömmes, P, Weiser, T, Hübscher, U
Format Journal Article
LanguageEnglish
Published United States 01.05.1990
Subjects
Animals
Antibody Specificity
Chickens - immunology
Egg Yolk
Immunization Schedule
Immunoglobulins - biosynthesis
Immunoglobulins - isolation & purification
Immunologic Techniques
Nuclear Proteins - immunology
Proliferating Cell Nuclear Antigen
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Summary:The egg yolk of immunized chicken is a rich and inexpensive source of specific polyclonal antibodies. In this paper we show that 20-30 micrograms of a highly conserved mammalian protein, as exemplified by proliferating cell nuclear antigen, are sufficient to induce an immune response. Immunoblot analysis revealed that specific antibodies appeared 20 days after immunization, reached a plateau after 30 days, and remained high until at least day 81. A total amount of 4 g immunoglobulin was extracted from 62 eggs of one immunized hen, yielding approximately 130 mg of specific antibodies.
ISSN:0892-6638
DOI:10.1096/fasebj.4.8.1970792