Centromeric DNA from chromosome VI in Saccharomyces cerevisiae strains

The functional sequence from the centromere in chromosome VI (CEN6) of Saccharomyces cerevisiae was narrowed down to a stretch of 500 bp by a Bal31 deletion approach. The DNA sequence in this region shows three long stretches, 40 bp, 96 bp, and 63 bp of 85% and more AT pairs and a pyrimidine purine...

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Bibliographic Details
Published inThe EMBO journal Vol. 1; no. 12; pp. 1605 - 1611
Main Authors Panzeri, L., Philippsen, P.
Format Journal Article
LanguageEnglish
Published England 01.01.1982
Subjects
Base Sequence
Chromosome Deletion
Chromosomes - analysis
DNA Restriction Enzymes
DNA, Fungal - genetics
Escherichia coli - genetics
Mutation
Plasmids
Saccharomyces cerevisiae
Saccharomyces cerevisiae - genetics
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Summary:The functional sequence from the centromere in chromosome VI (CEN6) of Saccharomyces cerevisiae was narrowed down to a stretch of 500 bp by a Bal31 deletion approach. The DNA sequence in this region shows three long stretches, 40 bp, 96 bp, and 63 bp of 85% and more AT pairs and a pyrimidine purine bias in the individual single strands. We assume that the CEN6 functional sequences encompass these AT‐rich stretches because this part shows striking similarities to sequence elements common to CEN3 and CEN11 DNA. A strain comparison revealed that CEN6 DNA sequences are confined to the Saccharomyces genus and probably only to the S. cerevisiae species. CEN6 is not highly conserved within S. cerevisiae strains because EcoRI and HindIII restriction site variants are found with high frequency.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
content type line 23
ISSN:0261-4189
1460-2075
DOI:10.1002/j.1460-2075.1982.tb01362.x