Isolation and identification of four flavonoid constituents from the seeds of Oroxylum indicum by high-speed counter-current chromatography

• Published in: Journal of Chromatography A Vol. 988; no. 1; pp. 95 - 105
• Main Authors: Chen, Li-Juan, Games, David E., Jones, Jonathan
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 21.02.2003
• Subjects: Bignoniaceae - chemistry; Countercurrent Distribution - methods; Flavonoids; Flavonoids - chemistry; Flavonoids - isolation & purification; Magnetic Resonance Spectroscopy; Seeds - chemistry; Oroxylum indicum; Plant materials; Flavonoids; Counter-current chromatography
• ISSN: 0021-9673
• DOI: 10.1016/S0021-9673(02)01954-4

Four flavonoids, chrysin, baicalein, baicalein-7- O-glucoside, baicalein-7- O-diglucoside (Oroxylin B) and one unknown flavonoid have been isolated and purified for the first time in the seeds of Oroxylum indicum by high-speed counter-current chromatography with a two-phase solvent system composed of chloroform–methanol–water (8:10:5, v/v). Then, 50 mg baicalein-7- O-glucoside, 10.5 mg baicalein-7- O-diglucoside, 4.5 mg chrysin-7- O-diglucoside, 25 mg baicalein and 45 mg chrysin could be obtained after injecting 20 mg/ml sample extract ten times and their purities were 96, 90, 85, 95 and 98%, respectively. All these constituents were identified by high-performance liquid chromatography–mass spectrometry and nuclear magnetic resonance.