Optical tweezers study of red blood cell aggregation and disaggregation in plasma and protein solutions

Kinetics of optical tweezers (OT)-induced spontaneous aggregation and disaggregation of red blood cells (RBCs) were studied at the level of cell doublets to assess RBC interaction mechanics. Measurements were performed under in vitro conditions in plasma and fibrinogen and fibrinogen + albumin solut...

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Published inJournal of biomedical optics Vol. 21; no. 3; p. 035001
Main Authors Lee, Kisung, Kinnunen, Matti, Khokhlova, Maria D, Lyubin, Evgeny V, Priezzhev, Alexander V, Meglinski, Igor, Fedyanin, Andrey A
Format Journal Article
LanguageEnglish
Published United States Society of Photo-Optical Instrumentation Engineers 01.03.2016
Subjects
Adult
Albumins - pharmacology
Equipment Design
Erythrocyte Aggregation - drug effects
Erythrocytes - cytology
Erythrocytes - drug effects
Erythrocytes - physiology
Fibrinogen - pharmacology
Humans
Male
Optical Tweezers
Plasma - chemistry
Young Adult
fibrinogen
blood microcirculation
albumin
cells interaction
optical tweezers
aggregation
red blood cell
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Summary:Kinetics of optical tweezers (OT)-induced spontaneous aggregation and disaggregation of red blood cells (RBCs) were studied at the level of cell doublets to assess RBC interaction mechanics. Measurements were performed under in vitro conditions in plasma and fibrinogen and fibrinogen + albumin solutions. The RBC spontaneous aggregation kinetics was found to exhibit different behavior depending on the cell environment. In contrast, the RBC disaggregation kinetics was similar in all solutions qualitatively and quantitatively, demonstrating a significant contribution of the studied proteins to the process. The impact of the study on assessing RBC interaction mechanics and the protein contribution to the reversible RBC aggregation process is discussed.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
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ISSN:1083-3668
1560-2281
DOI:10.1117/1.JBO.21.3.035001