Isolation and genomic characterization of a novel avian orthoreovirus strain in Korea, 2014

• Published in: Archives of virology Vol. 163; no. 5; pp. 1307 - 1316
• Main Authors: Noh, Jin-Yong, Lee, Dong-Hun, Lim, Tae-Hyun, Lee, Ji-ho, Day, J. Michael, Song, Chang-Seon
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01.05.2018; Springer Nature B.V
• Subjects: Animals; Arthritis; Arthritis, Infectious - epidemiology; Biomedical and Life Sciences; Biomedicine; Bird Diseases - epidemiology; Bird Diseases - virology; Brief Report; Chickens - virology; Genes, Viral; Genetic analysis; Genome, Viral; Genomes; Genotype; Genotypes; Infectious Diseases; Medical Microbiology; Mutation; Nucleotide sequence; Open Reading Frames; Orthoreovirus, Avian - classification; Orthoreovirus, Avian - genetics; Orthoreovirus, Avian - isolation & purification; Phylogeny; Point Mutation; Poultry; Reassortant Viruses - genetics; Recombination; Recombination, Genetic; Reoviridae Infections - veterinary; Reoviridae Infections - virology; Republic of Korea; Sequence Analysis, DNA; Virology; Intra-segmental recombination; Avian reovirus; Reassortment; Genetic analysis; Point mutation
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-017-3667-8

In this study, we isolated a novel avian reovirus (ARV) strain, K738/14, from a broiler chicken with viral arthritis in South Korea. Genome sequence comparisons showed relatively low nucleotide identity with previously identified ARV strains. Phylogenetic analyses suggested multiple reassortment events between reovirus strain S1133 and reoviruses of Hungarian, Chinese, and US origin had occurred. In addition, recombination analyses showed evidence of intra-segmental recombination in the M2 and S2 genes. Based on our genetic analyses, multiple reassortment events, intra-segmental recombination, and accumulation of point mutations have possibly contributed to the emergence of this novel genotype of ARV, identified in Korea.