Biofilm Growth on Simulated Fracture Fixation Plates Using a Customized CDC Biofilm Reactor for a Sheep Model of Biofilm-Related Infection

Most animal models of infection utilize planktonic bacteria as initial inocula. However, this may not accurately mimic scenarios where bacteria in the biofilm phenotype contaminate a site at the point of injury. We developed a modified CDC biofilm reactor in which biofilms can be grown on the surfac...

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Bibliographic Details
Published inMicroorganisms (Basel) Vol. 10; no. 4; p. 759
Main Authors Kay, Walker, Hunt, Connor, Nehring, Lisa, Barnum, Brian, Ashton, Nicholas, Williams, Dustin
Format Journal Article
LanguageEnglish
Published Switzerland MDPI AG 31.03.2022
MDPI
Subjects
Animal models
Antibiotics
Bacteria
biofilm
Biofilms
Bioreactors
Customization
customized
Drug resistance
Fixation
Metabolism
Methicillin
monomicrobial
MRSA
Open fractures
Phenotypes
Plates
polymicrobial
Pseudomonas aeruginosa
reactor
Reactors
Sheep
Simulation
Staphylococcus aureus
Staphylococcus infections
Surgeons
United States > US
S. aureus
customized
monomicrobial
MRSA
biofilm
reactor
P. aeruginosa
polymicrobial
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Summary:Most animal models of infection utilize planktonic bacteria as initial inocula. However, this may not accurately mimic scenarios where bacteria in the biofilm phenotype contaminate a site at the point of injury. We developed a modified CDC biofilm reactor in which biofilms can be grown on the surface of simulated fracture fixation plates. Multiple reactor runs were performed and demonstrated that monomicrobial biofilms of a clinical strain of methicillin-resistant , ATCC 6538, and ATCC 27853 consistently developed on fixation plates. We also identified a method by which to successfully grow polymicrobial biofilms of ATCC 6538 and ATCC 27853 on fixation plates. This customized reactor can be used to grow biofilms on simulated fracture fixation plates that can be inoculated in animal models of biofilm implant-related infection that, for example, mimic open fracture scenarios. The reactor provides a method for growing biofilms that can be used as initial inocula and potentially improve the testing and development of antibiofilm technologies.
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These authors contributed equally to this work.
ISSN:2076-2607
2076-2607
DOI:10.3390/microorganisms10040759