Luteolin Orchestrates Porcine Oocyte Meiotic Progression by Maintaining Organelle Dynamics Under Oxidative Stress
Increasing evidence has demonstrated that oxidative stress impairs oocyte maturation, but the underlying mechanisms remain largely unknown. Here, for the first time, we examined the antioxidant role of luteolin in meiotic progression and the underlying mechanisms. Supplementation of 5 μM luteolin in...
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| Published in | Frontiers in cell and developmental biology Vol. 9; p. 689826 |
|---|---|
| Main Authors | , , , , , , , , , |
| Format | Journal Article |
| Language | English |
| Published |
Frontiers Media S.A
15.06.2021
|
| Subjects | |
| Online Access | Get full text |
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| Summary: | Increasing evidence has demonstrated that oxidative stress impairs oocyte maturation, but the underlying mechanisms remain largely unknown. Here, for the first time, we examined the antioxidant role of luteolin in meiotic progression and the underlying mechanisms. Supplementation of 5 μM luteolin increased the rates of first polar body extrusion and blastocyst formation after parthenogenetic activation, and the expression levels of oocyte competence (
BMP15
and
GDF9
)-, mitogen-activated protein kinase (
MOS
)-, and maturation promoting factor (
CDK1
and
Cyclin B
)-related genes were also improved. Luteolin supplementation decreased intracellular reactive oxygen species levels and increased the expression levels of oxidative stress-related genes (
SOD1
,
SOD2
, and
CAT
). Interestingly, luteolin alleviated defects in cell organelles, including actin filaments, the spindle, mitochondria, the endoplasmic reticulum, and cortical granules, caused by H
2
O
2
exposure. Moreover, luteolin significantly improved the developmental competence of
in vitro
-fertilized embryos in terms of the cleavage rate, blastocyst formation rate, cell number, cellular survival rate, and gene expression and markedly restored the competencies decreased by H
2
O
2
treatment. These findings revealed that luteolin supplementation during
in vitro
maturation improves porcine meiotic progression and subsequent embryonic development by protecting various organelle dynamics against oxidative stress, potentially increasing our understanding of the underlying mechanisms governing the relationship between oxidative stress and the meiotic events required for successful oocyte maturation. |
|---|---|
| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Reviewed by: Shen Yin, Qingdao Agricultural University, China; Zhonghua Liu, Northeast Agricultural University, China This article was submitted to Cell Growth and Division, a section of the journal Frontiers in Cell and Developmental Biology These authors have contributed equally to this work and share first authorship Edited by: Heide Schatten, University of Missouri, United States |
| ISSN: | 2296-634X 2296-634X |
| DOI: | 10.3389/fcell.2021.689826 |