Prevalence, molecular epidemiology and zoonotic risk of Entamoeba spp. from experimental macaques in Yunnan Province, southwestern China

• Published in: Parasitology research (1987) Vol. 119; no. 8; pp. 2733 - 2740
• Main Authors: Pu, Li-Hua, Li, Zhao, Wu, Jie, Zhang, Yu-Lin, Chen, Jia-Qi, Yang, Jian-Fa, Zou, Feng-Cai
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.08.2020; Springer; Springer Nature B.V
• Subjects: Amebiasis; Animals; Animals, Laboratory; Biomedical and Life Sciences; Biomedicine; China - epidemiology; Diarrhea; DNA sequencing; DNA, Protozoan - genetics; Entamoeba; Entamoeba - classification; Entamoeba - genetics; Entamoeba - isolation & purification; Entamoebiasis - epidemiology; Entamoebiasis - parasitology; Entamoebiasis - transmission; Entamoebiasis - veterinary; Epidemiology; Feces - parasitology; Health aspects; Immunology; Infection; Macaca - parasitology; Medical Microbiology; Microbiology; Molecular Epidemiology; Nucleotide sequence; Nucleotide sequencing; Prevalence; Protozoan Infections, Animal - epidemiology; Protozoan Infections, Animal - parasitology; Protozoan Infections, Animal - transmission; Protozoology - Short Communication; Ribosome Subunits, Small - genetics; Risk factors; RNA; RNA, Ribosomal - genetics; rRNA; Sequence analysis; Sequence Analysis, DNA; Species; Zoonoses; China; spp; Prevalence; SSU rRNA; Nonhuman primates; Entamoeba spp
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-020-06762-9

Amebiasis is a worldwide parasitic zoonosis, with symptoms of abdominal discomfort, indigestion, diarrhea, and even death. However, limited information about the prevalence of Entamoeba spp. in experimental nonhuman primates (NHPs) in southwestern China is available. The objective of the current study was to investigate the frequency and species identity of Entamoeba to evaluate potential zoonotic risk factors for Entamoeba spp. infection in experimental NHPs. A total of 505 fecal samples were collected from NHPs (macaques) and analyzed by PCR analysis the small subunit rRNA (SSU rRNA) gene of Entamoeba spp. Forty-seven specimens were positive for Entamoeba spp., and the prevalence of Entamoeba spp. was 9.31% (47/505). Significant differences in the prevalence rates among the three breeds ( P =  0.002 < 0.01, df  = 2, χ 2  = 12.33) and feed types ( P =  0.001 < 0.01, df  = 1, χ 2  = 10.12) were observed. Altogether, four Entamoeba species, including E. dispar (57.44%), E. chattoni (29.78%), E. histolytica (6.38%), and E. coli (6.38%), were identified by DNA sequence analysis. The results suggested a low prevalence but high diversity of Entamoeba species in experimental NHPs in Yunnan Province, southwestern China. Results of this study contribute to the knowledge of the genetic characteristics of Entamoeba spp. in NHPs.