In-vitro scolicidal activity of Mallotus philippinensis (Lam.) Muell Arg. fruit glandular hair extract against hydatid cyst Echinococcus granulosus

• Published in: Asian Pacific journal of tropical medicine Vol. 6; no. 8; pp. 595 - 601
• Main Authors: Gangwar, Mayank, Verma, Vijay C, Singh, Tryambak D, Singh, Sushil K, Goel, RK, Nath, Gopal
• Format: Journal Article
• Language: English
• Published: India Elsevier B.V 01.08.2013; Department of Microbiology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005 UP India%Department of Medicinal Chemistry, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005 UP India%Department of Pharmaceutical Engineering, Institute of Technology, Banaras Hindu University, Varanasi-221005 UP India%Department of Pharmacology, Institute of Medical Sciences, Banaras Hindu University, Varanasi-221005 UP India
• Subjects: agents; Animals; Anthelmintics - isolation & purification; Anthelmintics - pharmacology; Anti-helminthic; Anti-helminthic agents; Biological Assay; blue; Echinococcus; Echinococcus granulosus; Echinococcus granulosus - drug effects; Fruit - chemistry; Hydatidosis; Mallotus; Mallotus Plant - chemistry; Mallotussp; Plant Extracts - isolation & purification; Plant Extracts - pharmacology; Praziquantel - pharmacology; Protoscolicidal; Survival Analysis; Trypan; Trypan blue; Anti-helminthic agents; Protoscolicidal; Echinococcus; Hydatidosis; Mallotussp; Trypan blue; Mallotus sp
• ISSN: 1995-7645; 2352-4146; 2352-4146
• DOI: 10.1016/S1995-7645(13)60103-0

Objective: To investigate new scolicidal agent from natural resources to cope with the side effects associated with synthetic drugs in Echinococcosis. Methods: The scolicidal potential of methanolic fruit powder extract (10 and 20 mg/mL) of Mallotus philippinensis ( M. philippinensis ) was investigated. Viability of protoscoleces was confirmed by trypan blue exclusion method, where mortality was observed at concentration of 10 and 20 mg/mL in 60 min treatment against Echinococcus granulosus ( E. granulosus ), under in-vitro conditions with reference to the known standard drug Praziquantel . Results: At concentration 10 and 20 mg/mL, the mortality rate was observed 97% and 99% respectively for 60 min treatment; while up to 93% mortality was observed with 20 mg/mL for only 10 min treatment. The concentration above 20 mg/mL for above 2 h showed 100% mortality, irrespective of further incubation. Conclusions: As compared with the standard anti-parasitic drug Praziquantel our extract has significant scolicidal activity with almost no associated side effects.