Phenotypicand Genotypic Characterization of Clinical Isolates of Intracellular Adherent–Invasive Escherichia coli Among Different Stages, Family History, and Treated Colorectal Cancer Patients in Iran
There is increasing evidence showing that microbial dysbiosis impacts the health and cancer risk of the host. An association between adherent–invasive Escherichia coli (AIEC) and colorectal cancer (CRC) has been revealed. Cyclomodulins (CMs) have been receiving increasing attention for carcinogenic...
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Published in | Frontiers in cellular and infection microbiology Vol. 12; p. 938477 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
Frontiers Media S.A
11.07.2022
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Subjects | |
Online Access | Get full text |
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Summary: | There is increasing evidence showing that microbial dysbiosis impacts the health and cancer risk of the host. An association between adherent–invasive
Escherichia coli
(AIEC) and colorectal cancer (CRC) has been revealed. Cyclomodulins (CMs) have been receiving increasing attention for carcinogenic changes. In this study, the incidence and features of intracellular AIEC and cyclomodulin-encoding genes were investigated and the phylogenetic grouping and genetic relatedness were evaluated.
E. coli
strains were isolated from the colorectal biopsies. Adhesion and invasion assays and intramacrophage cell survival test were performed to separate the AIEC isolates. Virulence genotyping for the genes
htrA
,
dsbA
,
chuA
, and
lpfA
and the cyclomodulin toxins was also conducted. In addition, phylogenetic grouping of the isolates was determined. Subsequently, repetitive element sequence-based PCR (rep-PCR) fingerprinting was performed. A total of 24 AIEC pathovars were isolated from 150 patients. The prevalence rates of
htr
,
dsbA
, and
lpfA
were 70.83% and that of
chuA
was 91.66%. The frequencies of the cyclomodulin toxins were as follows:
cnf1
, 29.2%;
cnf2
, 25%; colibactin, 29.2%; and
cdt
, 4.2%;
cif
was not found. Among the AIEC isolates, 4.2%, 4.2%, 54.2%, 29.2%, and 8.3% with phylotypes A or C, B1, B2, D, and E were identified, respectively. Left-sided colon carcinoma and adenocarcinoma T≥1 stage (CRC2) were colonized by B2 phylogroup AIEC-producing CMs more often than the samples from the other groups. Close genetic relatedness was observed in AIEC isolates with rep-PCR. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This article was submitted to Clinical Microbiology, a section of the journal Frontiers in Cellular and Infection Microbiology Reviewed by: Roberto Mauricio Vidal, University of Chile, Chile; Serena Schippa, Sapienza University of Rome, Italy; Ye Yang, University of Florida, United States; Dharmendra Kumar Soni, Uniformed Services University of the Health Sciences, United States Edited by: Shyamala Thirunavukkarasu, Washington University in St. Louis, United States |
ISSN: | 2235-2988 2235-2988 |
DOI: | 10.3389/fcimb.2022.938477 |