Down-regulated lncRNA ROR in tumor-educated platelets as a liquid-biopsy biomarker for nasopharyngeal carcinoma

• Published in: Journal of cancer research and clinical oncology Vol. 149; no. 8; pp. 4403 - 4409
• Main Authors: Wei, Jiazhou, Meng, Xian, Wei, Xiuqi, Zhu, Kaidong, Du, Li, Wang, Hui
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 01.07.2023; Springer Nature B.V
• Subjects: Biomarkers; Biopsy; Blood Platelets - metabolism; Cancer Research; carcinoma; Deoxyribonucleic acid; disease diagnosis; DNA; DNA viruses; DNA, Viral; Epstein-Barr virus; Epstein-Barr Virus Infections; Hematology; Herpesvirus 4, Human - genetics; Human gammaherpesvirus 4; Humans; Internal Medicine; Medicine; Medicine & Public Health; Nasopharyngeal carcinoma; Nasopharyngeal Carcinoma - diagnosis; Nasopharyngeal Carcinoma - genetics; Nasopharyngeal Neoplasms - diagnosis; Non-coding RNA; Oncology; Platelets; quantitative polymerase chain reaction; RNA, Long Noncoding - genetics; Throat cancer; Tumors; Nasopharyngeal carcinoma; Liquid biopsy; Diagnosis; lncRNA ROR; Tumor-educated platelets; Epstein–Barr virus
• ISSN: 0171-5216; 1432-1335; 1432-1335
• DOI: 10.1007/s00432-022-04350-1

Purposes To evaluate the diagnostic value of tumor-educated platelets (TEP) lncRNA ROR for nasopharyngeal carcinoma (NPC). Methods Quantitative real-time PCR was used to determine the expression level of TEP lncRNA ROR in NPC patients ( n  = 50) as compared to normal subjects ( n  = 33). The ROC curve analysis was performed to assess the diagnostic value of TEP lncRNA ROR for NPC. Correlations between TEP lncRNA ROR and clinical parameters were further analyzed. Results The median of TEP lncRNA ROR was significantly lower in NPC patients than that in normal subjects (0.0209 vs 0.0610, p  = 0.0019), while no significant difference was found in plasma lncRNA ROR. ROC analysis showed that TEP lncRNA ROR had a sensitivity of 60%, specificity of 70%, and accuracy of 63.9% in diagnosing NPC, and the area under ROC curve (AUC) was 0.70. The expression level of TEP lncRNA ROR in NPC showed no significant difference among different TNM stages. However, low level of TEP lncRNA ROR correlated well with positive Epstein–Barr virus (EBV) DNA (kappa value = 0.314, p  = 0.06), TEP lncRNA ROR and EBV DNA had similar diagnostic positive rate (58.3%) for NPC, and the combination of TEP lncRNA ROR and EBV DNA increased the positive rate to 74%. Conclusion The expression level of TEP lncRNA ROR was down-regulated in NPC and the diagnostic value of TEP lncRNA ROR was similar to EBV DNA. Our study indicated that TEP lncRNA ROR might serve as a novel type of liquid biopsy biomarker in diagnosis of NPC patients.