Genetic diversity of Streptomyces spp. causing common scab of potato in eastern Canada

• Published in: Systematic and applied microbiology Vol. 31; no. 6; pp. 474 - 484
• Main Authors: St-Onge, Renée, Goyer, Claudia, Coffin, Robert, Filion, Martin
• Format: Journal Article
• Language: English
• Published: München Elsevier GmbH 01.12.2008; Elsevier
• Subjects: 16S rRNA; bacterial diseases of plants; Bacterial plant pathogens; Bacterial Proteins - genetics; Bacteriology; Biological and medical sciences; Canada; Common scab; DNA Fingerprinting; Fundamental and applied biological sciences. Psychology; Genes, Bacterial; Genetic diversity; Genetic Variation; Genetics; Genomic Islands - genetics; geographical distribution; Melanins - genetics; Microbiology; Molecular Sequence Data; molecular systematics; Phenotype; phylogeny; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - microbiology; plant pathogenic bacteria; Plant Tubers - microbiology; Polymerase Chain Reaction; Polymorphism, Genetic; Potato; potatoes; Rep-PCR; ribosomal RNA; RNA, Ribosomal, 16S - genetics; RpoB; sequence analysis; Solanum tuberosum; Solanum tuberosum - microbiology; Streptomyces; Streptomyces - classification; Streptomyces - genetics; Streptomyces - isolation & purification; Streptomyces - pathogenicity; Streptomyces acidiscabies; Streptomyces scabies; Streptomyces spp; Systematics. Structure, properties and multiplication. Genetics; TomA; TxtA; TxtC; New Brunswick; Canada; Prince Edward Island; Nova Scotia; Potato; Common scab; 16S rRNA; TxtC; TxtA; Streptomyces spp; Rep-PCR; RpoB; TomA; Genetic diversity; Plant pathogen; Actinomycetales; Dicotyledones; Angiospermae; Molecular epidemiology; 16S-RNA; Bacteria; Solanaceae; Eastern Canada; Streptomycetaceae; Solanum tuberosum; Streptomyces; Polymerase chain reaction; Actinomycetes; Spermatophyta; Tuber plant
• ISSN: 0723-2020; 1618-0984
• DOI: 10.1016/j.syapm.2008.09.002

Common scab is an important disease of potato caused by Streptomyces scabies and other closely related species. In this study, the genetic diversity of Streptomyces spp. causing common scab of potato in eastern Canada was for the first time investigated. Forty-one Streptomyces spp. isolates were retrieved from necrotic lesions of potato tubers harvested from different regions of the Canadian provinces New-Brunswick, Nova Scotia and Prince-Edward-Island. Most isolates were closely related to known pathogenic S. scabies strains on the basis of partial 16S ribosomal (r) RNA and rpoB gene sequence analyses. Two isolates were identified as pathogenic species of Streptomyces acidiscabies. To our knowledge, this species has never been previously isolated in these areas. Genome fingerprinting studies using repetitive elements (rep) polymerase chain reactions (PCR) revealed 10 distinct genetic groups in eastern Canada. The geographical distribution of the genetic groups was region-dependant. Pathogenicity- and virulence-related genes ( txtA, txtC, and tomA) were PCR-amplified from each isolate, and nucleotide sequence analysis of partial gene fragments revealed slight polymorphisms in both txtA and txtC genes. No genetic variation was noted in the partial tomA gene sequences.