Telomerase Activation and ATRX Mutations Are Independent Risk Factors for Metastatic Pheochromocytoma and Paraganglioma

• Published in: Clinical cancer research Vol. 25; no. 2; pp. 760 - 770
• Main Authors: Job, Sylvie, Draskovic, Irena, Burnichon, Nelly, Buffet, Alexandre, Cros, Jérôme, Lépine, Charles, Venisse, Annabelle, Robidel, Estelle, Verkarre, Virginie, Meatchi, Tchao, Sibony, Mathilde, Amar, Laurence, Bertherat, Jérôme, de Reyniès, Aurélien, Londoño-Vallejo, Arturo, Favier, Judith, Castro-Vega, Luis Jaime, Gimenez-Roqueplo, Anne-Paule
• Format: Journal Article
• Language: English
• Published: United States American Association for Cancer Research 15.01.2019
• Subjects: Cancer; Cell Transformation, Neoplastic - genetics; Cell Transformation, Neoplastic - metabolism; DNA Methylation; DNA Mutational Analysis; Enzyme Activation; Human health and pathology; Humans; Life Sciences; Mutation; Neoplasm Staging; Paraganglioma - genetics; Paraganglioma - metabolism; Paraganglioma - mortality; Paraganglioma - pathology; Pheochromocytoma - genetics; Pheochromocytoma - metabolism; Pheochromocytoma - mortality; Pheochromocytoma - pathology; Prognosis; Promoter Regions, Genetic; Telomerase - metabolism; Whole Genome Sequencing; X-linked Nuclear Protein - genetics; X-linked Nuclear Protein - metabolism
• ISSN: 1078-0432; 1557-3265
• DOI: 10.1158/1078-0432.ccr-18-0139

Pheochromocytomas and paragangliomas (PPGLs) are rare neuroendocrine tumors. Whereas most PPGLs are benign, up to 20% may become metastatic with - and -mutated tumors showing the higher risk. We aimed at determining the contribution of immortalization mechanisms to metastatic progression. Immortalization mechanisms were investigated in 200 tumors. To identify telomerase (+) tumors, we analyzed genomic alterations leading to transcriptional activation of comprising promoter mutations, hypermethylation and gain copy number. To identify tumors that activated the alternative lengthening of telomere (ALT) mechanism, we combined analyses of telomere length by slot blot, telomere heterogeneity by telomere FISH, and mutations by next-generation sequencing. Univariate/multivariate and metastasis-free survival (MFS) and overall survival (OS) analyses were carried out for assessment of risk factors and clinical outcomes. Only 37 of 200 (18.5%) tumors achieved immortalization. Telomerase activation occurred in 12 metastatic tumors and was prevalent in -mutated paragangliomas ( = 2.42e-09). ALT features were present in 25 tumors, mostly pheochromocytomas, regardless of metastatic status or molecular group ( = 0.169), yet mutations were found preferentially in -mutated metastatic tumors ( = 0.0014). Telomerase activation and mutations were independent factors of poor prognosis: MFS (hazard ratio, 48.2 and 33.1; = 6.50E-07 and 1.90E-07, respectively); OS (hazard ratio, 97.4 and 44.1; = 4.30E-03 and 2.00E-03, respectively) and were associated with worse MFS and OS (log-rank tests < 0.0001). Assessment of telomerase activation and mutations could be used to identify metastatic PPGLs, particularly in tumors at high risk of progression.