A therapeutic oxygen carrier isolated from Arenicola marina decreased P. gingivalis induced inflammation and tissue destruction

• Published in: Scientific reports Vol. 10; no. 1; p. 14745
• Main Authors: Batool, Fareeha, Stutz, Céline, Petit, Catherine, Benkirane-Jessel, Nadia, Delpy, Eric, Zal, Franck, Leize-Zal, Elisabeth, Huck, Olivier
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 08.09.2020; Nature Publishing Group
• Subjects: 692/420/254; 692/420/256; 692/699/3017; Animals; Anti-Inflammatory Agents - pharmacology; Bacteroidaceae Infections - complications; Bacteroidaceae Infections - drug therapy; Bacteroidaceae Infections - microbiology; Biofilms; Brain Abscess - drug therapy; Brain Abscess - microbiology; Brain Abscess - pathology; Chemokines; Cytokines; Cytotoxicity; Disease; Gene expression; Gingiva - chemistry; Gingiva - microbiology; Growth factors; Hemoglobin; Human health and pathology; Humanities and Social Sciences; Humans; Infections; Inflammation; Inflammation - drug therapy; Inflammation - microbiology; Inflammation - pathology; Ischemia; Life Sciences; Male; Mice; Mice, Inbred C57BL; Molecular weight; multidisciplinary; Oxygen - pharmacology; Oxygenation; Polychaeta - chemistry; Porphyromonas gingivalis - drug effects; Porphyromonas gingivalis - growth & development; Porphyromonas gingivalis - isolation & purification; Science; Science (multidisciplinary); Signal Transduction; Skull - drug effects; Skull - microbiology; Skull - pathology; Thermocline; Transforming growth factor-b1; Tumor necrosis factor-TNF; Wound healing; South China Sea
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-020-71593-8

The control of inflammation and infection is crucial for periodontal wound healing and regeneration. M101, an oxygen carrier derived from Arenicola marina, was tested for its anti-inflammatory and anti-infectious potential based on its anti-oxidative and tissue oxygenation properties. In vitro, no cytotoxicity was observed in oral epithelial cells (EC) treated with M101. M101 (1 g/L) reduced significantly the gene expression of pro-inflammatory markers such as TNF-α, NF-κΒ and RANKL in P. gingivalis -LPS stimulated and P. gingivalis -infected EC. The proteome array revealed significant down-regulation of pro-inflammatory cytokines (IL-1β and IL-8) and chemokine ligands (RANTES and IP-10), and upregulation of pro-healing mediators (PDGF-BB, TGF-β1, IL-10, IL-2, IL-4, IL-11 and IL-15) and, extracellular and immune modulators (TIMP-2, M-CSF and ICAM-1). M101 significantly increased the gene expression of Resolvin-E1 receptor. Furthermore, M101 treatment reduced P. gingivalis biofilm growth over glass surface, observed with live/dead analysis and by decreased P. gingivalis 16 s rRNA expression (51.7%) ( p  <  0.05) . In mice , M101 reduced the clinical abscess size (50.2%) in P. gingivalis -induced calvarial lesion concomitant with a decreased inflammatory score evaluated through histomorphometric analysis, thus, improving soft tissue and bone healing response. Therefore, M101 may be a novel therapeutic agent that could be beneficial in the management of P. gingivalis associated diseases.