Thyroid Hormone Activates Protein Arginine Methyltransferase 1 Expression by Directly Inducing c-Myc Transcription during Xenopus Intestinal Stem Cell Development

• Published in: The Journal of biological chemistry Vol. 287; no. 13; pp. 10039 - 10050
• Main Authors: Fujimoto, Kenta, Matsuura, Kazuo, Hu-Wang, Eileen, Lu, Rosemary, Shi, Yun-Bo
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 23.03.2012; American Society for Biochemistry and Molecular Biology
• Subjects: Adult Stem Cells; Animals; Base Sequence; Caco-2 Cells; Gene Expression Regulation, Developmental - physiology; Gene Expression Regulation, Enzymologic - physiology; Gene Regulation; Humans; Intestinal Mucosa - metabolism; Intestine; Intestines - cytology; Intestines - embryology; Metamorphosis; Metamorphosis, Biological - physiology; Mice; Molecular Sequence Data; PRMT1; Promoter Regions, Genetic - physiology; Protein-Arginine N-Methyltransferases - biosynthesis; Protein-Arginine N-Methyltransferases - genetics; Proto-Oncogene Proteins c-myc - genetics; Proto-Oncogene Proteins c-myc - metabolism; Receptors, Thyroid Hormone - genetics; Receptors, Thyroid Hormone - metabolism; Stem Cells; Stem Cells - cytology; Stem Cells - metabolism; Thyroid Hormone; Thyroid Hormone Receptor; Transcription Coactivators; Transcription, Genetic - physiology; Triiodothyronine - genetics; Triiodothyronine - metabolism; Xenopus; Xenopus laevis; Xenopus Proteins - genetics; Xenopus Proteins - metabolism; Zebrafish - embryology; Zebrafish - genetics; Zebrafish - metabolism; Stem Cells; Transcription Coactivators; Xenopus; Thyroid Hormone; Adult Stem Cells; Intestine; PRMT1; Metamorphosis; Thyroid Hormone Receptor
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M111.335661

Adult organ-specific stem cells are essential for organ homeostasis and tissue repair and regeneration. The formation of such stem cells during vertebrate development is poorly understood. Intestinal remodeling during thyroid hormone (T3)-dependent Xenopus metamorphosis resembles postembryonic intestinal maturation in mammals. During metamorphosis, the intestine is remodeled de novo via a yet unknown mechanism. Protein arginine methyltransferase 1 (PRMT1) is up-regulated in and required for adult intestinal stem cells during metamorphosis. PRMT1 up-regulation is the earliest known molecular event for the developing stem cells and is also conserved during zebrafish and mouse intestinal development. To analyze how PRMT1 is specifically up-regulated during the formation of the adult intestinal stem cells, we cloned the Xenopus PRMT1 promoter and characterized it in CaCo-2 cells, a human cell line with intestinal stem cell characteristics. Through a series deletion and mutational analyses, we showed that the stem cell-associated transcription factor c-Myc could bind to a conserved site in the first intron to activate the promoter. Furthermore, we demonstrated that during metamorphosis, both c-Myc and PRMT1 were highly up-regulated, specifically in the remodeling intestine but not the resorbing tail, and that c-Myc was induced by T3 prior to PRMT1 up-regulation. In addition, we showed that T3 directly activated the c-Myc gene during metamorphosis in the intestine via binding of the T3 receptor to the c-Myc promoter. These results suggest that T3 induces c-Myc transcription directly in the intestine, that c-Myc, in turn, activates PRMT1 expression, and that this is an important gene regulation cascade controlling intestinal stem cell development. PRMT1 is highly up-regulated in and plays an important role for developing adult intestinal stem cells during thyroid hormone-dependent Xenopus metamorphosis. Liganded thyroid hormone receptor activates c-Myc transcription. c-Myc, in turn, activates PRMT1. Thyroid hormone up-regulates PRMT1 indirectly via c-Myc. The finding reveals an important gene regulation pathway by which thyroid hormone controls stem cell development.