Characterization of Salmonella Gallinarum isolates from backyard poultry by polymerase chain reaction detection of invasion ( inv A) and Salmonella plasmid virulence ( spv C) genes

• Published in: Veterinary World Vol. 10; no. 7; pp. 814 - 817
• Main Authors: Pal, Susmita, Dey, Samir, Batabyal, Kunal, Banerjee, Abhiroop, Joardar, Siddhartha Narayan, Samanta, Indranil, Isore, Devi Prasad
• Format: Journal Article
• Language: English
• Published: India Veterinary World 01.07.2017
• Subjects: Chickens; Conserved sequence; Fowl typhoid; Genes; Homology; invA; Nucleotide sequence; Outbreaks; Polymerase chain reaction; Poultry; Salmonella; Salmonella Gallinarum; Salmonella plasmid virulence C; Typhoid; Virulence; virulence genes; invA; Salmonella plasmid virulence C; Salmonella Gallinarum; polymerase chain reaction; virulence genes
• ISSN: 0972-8988; 2231-0916
• DOI: 10.14202/vetworld.2017.814-817

The aim was to characterize serovar Gallinarum isolated from backyard poultry by polymerase chain reaction (PCR) detection of virulence genes invasion ( A) and plasmid virulence C ( C). Two strains of serovar Gallinarum isolates used in this study were obtained from an outbreak of fowl typhoid in backyard Vanaraja fowl. PCR technique was used for detection of A and C genes using standard methodology. The A PCR product from one representative isolate was sequenced and compared with other related serovars in GenBank data. Gallinarum produced expected amplicons of A and C gene products. Nucleotide sequence of 285 bp A gene was deposited in GenBank with accession no. KX788214. Sequence analysis of A gene was found conserved in serovars and demonstrated 100% homology with closely related serovars of . Invasion gene ( A) was found to be highly conserved in Gallinarum and highly similar with closely related serovars. The isolates also contained plasmid-mediated spvC gene indicating possession of virulence plasmid.