Indole inhibited the expression of csrA gene in Escherichia coli

• Published in: Journal of general and applied microbiology Vol. 69; no. 5; pp. 239 - 248
• Main Authors: Zuo, Guocai, Shi, Zhenxia, Guo, Huiying, Sun, Zemin, Zheng, Jing, Feng, Yongjun, Zhou, Zhiguo
• Format: Journal Article
• Language: English
• Published: Japan Applied Microbiology, Molecular and Cellular Biosciences Research Foundation 01.01.2023; Japan Science and Technology Agency
• Subjects: csrA; E coli; Escherichia coli; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli Proteins - genetics; Escherichia coli Proteins - metabolism; Gene expression; Gene Expression Regulation, Bacterial; Glycogen; Glycogens; Indole; Indoles; Indoles - pharmacology; Regulation; Repressor Proteins - genetics; Repressor Proteins - metabolism; RNA-Binding Proteins - chemistry; RNA-Binding Proteins - genetics; RNA-Binding Proteins - metabolism; csrA; Regulation; Escherichia coli; Indole
• ISSN: 0022-1260; 1349-8037
• DOI: 10.2323/jgam.2023.06.007

Indole is a very important signal molecule which plays multiple regulatory roles in many physiological and biochemical processes of bacteria, but up to now, the reasons for its wide range of functions have not been revealed. In this study, we found that indole inhibits the motility, promotes glycogen accumulation and enhances starvation resistance of Escherichia coli. However, the regulatory effects of indole became insignificant while the global csrA gene was mutated. To reveal the regulatory relationship between indole and csrA, we studied the effects of indole on the transcription level of csrA, flhDC, glgCAP and cstA, and also the sensing of the promoters of the genes on indole. It was found that indole inhibited the transcription of csrA, and only the promoter of the csrA gene can sense indole. Namely, indole indirectly regulated the translation level of FlhDC, GlgCAP and CstA. These data indicates that indole regulation is related with the regulation of CsrA, which may throw light on the regulation mechanism research of indole.