Black soybean extract can attenuate thrombosis through inhibition of collagen-induced platelet activation

• Published in: The Journal of nutritional biochemistry Vol. 22; no. 10; pp. 964 - 970
• Main Authors: Kim, Keunyoung, Lim, Kyung-Min, Kim, Chae-Wook, Shin, Hyun-Jung, Seo, Dae-Bang, Lee, Sang-Jun, Noh, Ji-Yoon, Bae, Ok-Nam, Shin, Sue, Chung, Jin-Ho
• Format: Journal Article
• Language: English
• Published: New York, NY Elsevier Inc 01.10.2011; Elsevier
• Subjects: active ingredients; Adolescent; Adult; anticarcinogenic activity; Antiplatelet activity; Antithrombotic activity; Biological and medical sciences; Black soybean; Blood Platelets - drug effects; Blood Platelets - metabolism; clinical trials; Collagen; Collagen - metabolism; dietary supplements; Feeding. Feeding behavior; fractionation; Fundamental and applied biological sciences. Psychology; Glycine max; Glycine max - chemistry; Humans; Male; nuclear magnetic resonance spectroscopy; oral administration; P-Selectin - metabolism; Plant Extracts - pharmacology; Platelet Activation - drug effects; platelet aggregation; risk; secretion; serotonin; Serotonin - metabolism; soybeans; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization; thrombosis; Thrombosis - blood; Thrombosis - prevention & control; Vertebrates: anatomy and physiology, studies on body, several organs or systems; BB; Anti-CD62P-FITC Ab; IC 50; Antithrombotic activity; anti-GPIb-PE Ab; TxB 2; YB; Antiplatelet activity; PRP; PPP; Collagen; TxA 2; Black soybean; Glycoprotein; Cardiovascular disease; Activation; Extract; Black; Soybean; Thrombosis; Vascular disease; Vertebrata; Platelet; Mammalia; Inhibition
• ISSN: 0955-2863; 1873-4847
• DOI: 10.1016/j.jnutbio.2010.08.008

Many clinical trials have demonstrated the beneficial effects of soybean (Glycine max) on general cardiovascular health. Among a variety of soybeans, black soybean is known to display diverse biological activities superior to those of yellow and green soybeans, such as in antioxidant, anti-inflammatory and anticancer activities. However, few studies have been directed on the effect of black soybean on cardiovascular function. In this study, we aimed to investigate the effect of black soybean extract (BB) on platelet activation, a key contributor to thrombotic diseases. In freshly isolated human platelets, BB has shown potent inhibitory activity on collagen-induced platelet aggregation, while yellow soybean extract had marginal activity only. BB also attenuated serotonin secretion and P-selectin expression, which are important factors for the platelet–tissue interaction along with thromboxane A2 formation. These in vitro results were further confirmed in an ex vivo platelet aggregation measurement and in vivo venous thrombosis model where oral administration of BB reduced collagen-induced platelet aggregation and FeCl3-induced thrombus formation significantly. A potential active ingredient for antiplatelet effects of BB was isolated and identified to be adenosine through bioassay-directed fractionation and NMR and ESI-MS analyses. These results indicate that black soybean can be a novel dietary supplement for the prevention of cardiovascular risks and the improvement of blood circulation.