Relationship between LSD1 expression and E-cadherin expression in prostate cancer

• Published in: International urology and nephrology Vol. 47; no. 3; pp. 485 - 490
• Main Authors: Wang, Min, Liu, Xiuheng, Jiang, Guanjun, Chen, Hui, Guo, Jia, Weng, Xiaodong
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01.03.2015; Springer Nature B.V
• Subjects: Cadherins - analysis; Cadherins - drug effects; Carcinoma - chemistry; Carcinoma - secondary; Cell Line, Tumor; Disease Progression; Histone Demethylases - analysis; Histone Demethylases - antagonists & inhibitors; Humans; Male; Medicine; Medicine & Public Health; Neoplasm Grading; Neoplasm Metastasis; Nephrology; Pargyline - pharmacology; Prognosis; Prostatic Hyperplasia - metabolism; Prostatic Neoplasms - chemistry; Prostatic Neoplasms - pathology; Urology; Urology – Original Paper; Progression; E-cadherin; Prostate cancer; LSD1; Metastases
• ISSN: 0301-1623; 1573-2584
• DOI: 10.1007/s11255-015-0915-2

Purpose To investigate the relationship between the expression of LSD1 and E-cadherin in prostate cancer and their prognostic significance. Methods The expression of LSD1 and E-cadherin in prostate cancer was detected using immunohistochemistry, and the relationship between the expressions of these two molecules was analyzed by correlation analysis. Furthermore, LNCap cell line was treated with Pargyline (an inhibitor of LSD1), and Western blot was used to analyze LSD1 and E-cadherin expression. Results LSD1 expression increased significantly in prostate cancer specimens compared with benign prostatic hyperplasia ( P  < 0.05). Further analysis testified that LSD1 expression was positively correlated with higher Gleason Score, distant metastases, and poor prognosis ( P  < 0.05). Nevertheless, E-cadherin expression decreased significantly in prostate cancer specimens compared with benign prostatic hyperplasia ( P  < 0.05) and was negatively correlated with higher Gleason Score, distant metastases ( P  < 0.05). Correlation analysis revealed that LSD1 expression was negatively correlated with E-cadherin expression in prostate cancer ( rs  = −0.486, P  = 0.001). Positive LSD1 expression and negative E-cadherin expression were significantly correlated with high 2-year progression (occurrence of castration-resistant prostate cancer) rate and low 5-year survival rate ( P  < 0.05). Moreover, Pargyline inhibited activity of LSD1 and up-regulated E-cadherin expression. Conclusion High LSD1 expression combined with low E-cadherin expression might be predictors of prostate cancer progression and metastasis. Inhibition of LSD1 may be a potential therapeutic target for prevention of prostate cancer.