Physico-Chemical and Metagenomic Profile Analyses of Animal Manures Routinely Used as Inocula in Anaerobic Digestion for Biogas Production

• Published in: Microorganisms (Basel) Vol. 10; no. 4; p. 671
• Main Authors: Mutungwazi, Asheal, Ijoma, Grace N, Ogola, Henry J O, Matambo, Tonderayi S
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 22.03.2022; MDPI
• Subjects: Agricultural production; Anaerobic digestion; Animal wastes; Animals; autochthonous; Biogas; Bioinformatics; Carbon; Catalysis; Consortia; Energy shortages; Enzymes; feed composition; Feeds; Gastrointestinal system; Gastrointestinal tract; Horse manure; Horses; Inoculation; Manures; Metabolic pathways; Metagenomics; Methane; Microbial activity; microbiome; Microorganisms; Nitrogen; nutrient utilisation; Organic wastes; Waste management; United States > US; South Africa; feed composition; gastrointestinal tract; nutrient utilisation; autochthonous; microbiome
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms10040671

Anaerobic digestion (AD) of organic waste is considered a sustainable solution to energy shortage and waste management challenges. The process is facilitated by complex communities of micro-organisms, yet most wastes do not have these and thus need microbial inoculation using animal manures to initiate the process. However, the degradation efficiency and methane yield achieved in using different inocula vary due to their different microbial diversities. This study used metagenomics tools to compare the autochthonous microbial composition of cow, pig, chicken, and horse manures commonly used for biogas production. Cows exhibited the highest carbon utilisation (>30%) and showed a carbon to nitrogen ratio (C/N) favourable for microbial growth. Pigs showed the least nitrogen utilisation (<3%) which explains their low C/N whilst horses showed the highest nitrogen utilisation (>40%), which explains its high C/N above the optimal range of 20−30 for efficient AD. Manures from animals with similar gastrointestinal tract (GIT) physiologies were observed to largely harbour similar microbial communities. Conversely, some samples from animals with different GITs also shared common microbial communities plausibly because of similar diets and rearing conditions. Insights from this study will lay a foundation upon which in-depth studies of AD metabolic pathways and strategies to boost methane production through efficient catalysis can be derived.