Effects of LAB Inoculants on the Fermentation Quality, Chemical Composition, and Bacterial Community of Oat Silage on the Qinghai-Tibetan Plateau

• Published in: Microorganisms (Basel) Vol. 10; no. 4; p. 787
• Main Authors: Cheng, Qiming, Chen, Liangyin, Chen, Yulian, Li, Ping, Chen, Chao
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 08.04.2022; MDPI
• Subjects: Acetic acid; Additives; Ambient temperature; Ammonia; Antiinfectives and antibacterials; Bacteria; bacterial community; Butyric acid; Chemical composition; Cold; Cold regions; Community composition; Deterioration; Dry matter; Fermentation; Inoculation; Laboratories; Lactic acid; Lactic acid bacteria; Lactiplantibacillus plantarum; Leuconostoc; Low temperature; Metabolites; Microorganisms; phenyllactic acid; Relative abundance; Silage; Software; Temperature tolerance; Beijing China; Tibetan Plateau; China; fermentation; bacterial community; phenyllactic acid; lactic acid bacteria; silage
• ISSN: 2076-2607; 2076-2607
• DOI: 10.3390/microorganisms10040787

Lactic acid bacteria (LAB) have been proposed for the control of undesirable fermentation and, subsequently, aerobic deterioration due to their ability to produce antimicrobial metabolites in silage mass. To investigate the effect of specific LAB on the silage fermentation characteristics and bacterial community composition of oat in cold regions, silages were treated without (control) or with three LAB strains (LB, Lentilactobacillus buchneri; nLP, low temperature tolerant Lactiplantibacillus plantarum; pLP, phenyllactic acid-producing Lactiplantibacillus plantarum), and then stored at ambient temperature (−2.63 ± 5.47−14.29 ± 5.48 °C) for 30, 60, and 90 days. Compared with control, inoculation of LAB decreased the final pH value, butyric acid content, ammonia-N of total N and dry matter loss of silage. Treatments with nLP and pLP increased (p < 0.05) lactic acid content, whereas LB increased (p < 0.05) acetic acid content of silage. Lactiplantibacillus and Leuconostoc dominated in the silages with relative abundance of 68.29−96.63%. A prolonged storage period enhanced the growth of Leuconostoc in pLP-treated silage. In addition, pLP increased (p < 0.05) the aerobic stability of silage as compared with nLP. In conclusion, inoculation of LAB improved silage fermentation and/or delayed aerobic deterioration by shifting bacterial community composition during ensiling. Phenyllactic acid-producing Lactiplantibacillusplantarum as an inoculant exhibited potential for high quality silage production.