The Shuttling SR Protein 9G8 Plays a Role in Translation of Unspliced mRNA Containing a Constitutive Transport Element

• Published in: The Journal of biological chemistry Vol. 282; no. 27; pp. 19844 - 19853
• Main Authors: Swartz, Jennifer E., Bor, Yeou-Cherng, Misawa, Yukiko, Rekosh, David, Hammarskjold, Marie-Louise
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 06.07.2007; American Society for Biochemistry and Molecular Biology
• Subjects: Active Transport, Cell Nucleus - physiology; Cell Line; Cell Nucleus - metabolism; Exons - physiology; Humans; Mason-Pfizer monkey virus; Mason-Pfizer monkey virus - metabolism; Models, Biological; Nuclear Proteins; Nucleocytoplasmic Transport Proteins - metabolism; Peptide Chain Initiation, Translational - physiology; Phosphorylation; Polyribosomes - metabolism; Protein Processing, Post-Translational; RNA Splicing - physiology; RNA, Viral - metabolism; RNA-Binding Proteins - metabolism; Serine-Arginine Splicing Factors
• ISSN: 0021-9258; 1083-351X
• DOI: 10.1074/jbc.M701660200

The splicing regulatory SR protein, 9G8, has recently been proposed to function in mRNA export in conjunction with the export protein, Tap/NXF1. Tap interacts directly with the Mason-Pfizer monkey virus constitutive transport element (CTE), an element that enables export of unspliced, intron-containing mRNA. Based on our previous finding that Tap can promote polysome association and translation of CTE-RNA, we investigated the effect of 9G8 on cytoplasmic RNA fate. 9G8 was shown to enhance expression of unspliced RNA containing either the Mason-Pfizer monkey virus-CTE or the recently discovered Tap-CTE. 9G8 also enhanced polyribosome association of unspliced RNA containing a CTE. Hyperphosphorylated 9G8 was present in monosomes and small polyribosomes, whereas soluble fractions contained only hypophosphorylated protein. Our results are consistent with a model in which hypophosphorylated SR proteins remain stably associated with messenger ribonucleoprotein (mRNP) complexes during export and are released during translation initiation concomitant with increased phosphorylation. These results provide further evidence for crucial links between RNA splicing, export and translation.