Polymeric lamellar substrate particles as carrier adjuvant for recombinant hepatitis B surface antigen vaccine

• Published in: Vaccine Vol. 27; no. 17; pp. 2372 - 2378
• Main Authors: Saini, Vinay, Sahoo, M.K, Murthy, P.K, Kohli, Dharmveer
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 14.04.2009; Elsevier; Elsevier Limited
• Subjects: Adjuvants, Immunologic - administration & dosage; Adjuvants, Immunologic - physiology; Allergy and Immunology; Animals; Antibody Formation; Applied microbiology; Atoms & subatomic particles; Biological and medical sciences; Drug Administration Schedule; Drug Carriers - administration & dosage; Fundamental and applied biological sciences. Psychology; Hepatitis; Hepatitis B surface antigen; Hepatitis B Surface Antigens - administration & dosage; Hepatitis B Surface Antigens - immunology; Hepatitis B Vaccines - administration & dosage; Hepatitis B Vaccines - immunology; Hepatitis B, Chronic - immunology; Hepatitis B, Chronic - prevention & control; Humans; IFN-γ; IgG; Immune system; Interferon-gamma - biosynthesis; Lymphocyte Activation; Lymphocytes - immunology; Lymphocytes - metabolism; Lymphoproliferation; Macrophages, Peritoneal - immunology; Macrophages, Peritoneal - metabolism; Mice; Microbiology; Microspheres; Miscellaneous; Nitric Oxide - biosynthesis; PLSP; Polymers - chemistry; Vaccines; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects); Vaccines, Synthetic - administration & dosage; Vaccines, Synthetic - immunology; Viral infections; Virology; India; IgG; NO; IFN-γ; Hepatitis B surface antigen; PLSP; Lymphoproliferation; Cytokine; Orthohepadnavirus; Vaccine; Virus; Hepadnaviridae; Immunological adjuvant; Recombinant protein; Hepatitis B virus; Gamma interferon
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/j.vaccine.2009.02.023

Abstract Blank polymeric lamellar substrate particles (PLSP) of poly ( l -lactide) were prepared and recombinant hepatitis B surface antigen (rHBsAg) was adsorbed onto these particles. The physical characteristics of blank PLSPs or PLSP-rHBsAg in vitro and its immunological responses in Balb/c mice were investigated. The average size of the particles was less than 10 μm. Antigen adsorption efficiency was found to be 62.66 ± 1.26%. Immunization with PLSP-rHBsAg resulted in upregulation of specific cellular (lymphoproliferation, IFN-γ and NO release) as well as IgG response in animals. These responses were higher than those produced by two-dose schedule of alum-adsorbed antigen (alum-rHBsAg). Thus in conclusion, in terms of convenience and efficacy PLSP-rHBsAg is superior to alum-rHBsAg.