Barley anther culture: effects of annual cycle and spike position on microspore embryogenesis and albinism

• Published in: Plant cell reports Vol. 25; no. 5; pp. 375 - 381
• Main Authors: Jacquard, C, Asakaviciute, R, Hamalian, A.M, Sangwan, R.S, Devaux, P, Clement, C
• Format: Journal Article
• Language: English
• Published: Berlin Springer 01.05.2006; Springer Nature B.V
• Subjects: albino; anther culture; anthers; barley; Biological and medical sciences; Biotechnology; Crosses, Genetic; Culture Techniques; Eukaryotic cell cultures; Fundamental and applied biological sciences. Psychology; Germination; Hordeum - embryology; Hordeum - genetics; Hordeum - physiology; Hordeum vulgare; inflorescences; Methods. Procedures. Technologies; micropropagation; microspores; Miscellaneous; Plant cells and fungal cells; Plant Leaves - embryology; Plant Leaves - genetics; Plant Leaves - physiology; plant regeneration; plantlets; Seasons; Seeds - genetics; Seeds - physiology; somatic embryogenesis; somatic embryos; Spores - genetics; Spores - physiology; tillers; Embryonic development; Barley; Monocotyledones; Vegetals; Albinism; Hordeum vulgare; Anther culture; Androgenesis; Gramineae; Seasonal variation; Angiospermae; Spermatophyta; Tillering effect
• ISSN: 0721-7714; 1432-203X
• DOI: 10.1007/s00299-005-0070-9

The effect of donor plants annual cycle and anther/spike position on the production of microspore-derived plants and albinism were studied. We used the winter cv. Igri and the spring cv. Cork, known to respond similarly in anther culture but to produce 78% and 2% of green plants, respectively. In both cvs. the number of microspore-derived plants was significantly higher when the anthers were collected from January to July than from August to December. However, during this period the proportion of albino plants was not altered. Conversely, the anther response decreased from 76.6 to 31.5% in Igri and from 58.8 to 32.0% in Cork when the donor spike originates from the main shoot or the fourth tiller. Significantly, anthers collected from spike of the second tiller enabled us to drastically increase the proportion of regenerated green plantlets, by 16% in Igri and 1800% in Cork.