Genetic Characteristics and Immunogenicity of Pandemic H1N1 Influenza Virus Isolate from Pig in Korea

• Published in: Immune Network Vol. 16; no. 5; pp. 311 - 315
• Main Authors: Moon, Hyoung Joon, Oh, Jin Sik, Yeom, Minjoo, Han, Sang Yoon, Kim, Sung Jae, Park, Bong Kyun, Song, Dae Sub, Kang, Bo Kyu
• Format: Journal Article
• Language: English
• Published: Korea (South) The Korean Association of Immunobiologists 2016; 대한면역학회; The Korean Association of Immunologists
• Subjects: Brief Communication; 면역학; Vaccine; H1N1; Influenza; Pig
• ISSN: 1598-2629; 2092-6685
• DOI: 10.4110/in.2016.16.5.311

A pandemic influenza A (H1N1) virus strain was isolated from a pig farm in Korea in December 2009. The strain was propagated in and isolated from both the Madin-Darby canine kidney cell line and embryonated eggs. The partial and complete sequences of the strain were identical to those of A/California/04/2009, with >99% sequence similarity in the HA, NA, M, NS, NP, PA, PB1, and PB2 genes. The isolated strain was inactivated and used to prepare a swine influenza vaccine. This trial vaccine, containing the new isolate that has high sequence similarity with the pandemic influenza A (H1N1) virus, resulted in seroconversion in Guinea pigs and piglets. This strain could therefore be a potential vaccine candidate for swine influenza control in commercial farms.