Graphene-based carbon-layered electrode array technology for neural imaging and optogenetic applications

• Published in: Nature communications Vol. 5; no. 1; p. 5258
• Main Authors: Park, Dong-Wook, Schendel, Amelia A., Mikael, Solomon, Brodnick, Sarah K., Richner, Thomas J., Ness, Jared P., Hayat, Mohammed R., Atry, Farid, Frye, Seth T., Pashaie, Ramin, Thongpang, Sanitta, Ma, Zhenqiang, Williams, Justin C.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 20.10.2014; Nature Publishing Group; Nature Pub. Group
• Subjects: 14/63; 140/133; 142/126; 631/1647/2253; 631/378; 64/60; 64/86; 692/700/1421/65; 9/30; Animals; Biocompatible Materials - chemistry; Carbon - chemistry; Electrodes; Equipment Design; Female; Graphite - chemistry; Humanities and Social Sciences; Imaging, Three-Dimensional; Male; Mice; Microscopy, Fluorescence; multidisciplinary; Neuroimaging - instrumentation; Optics and Photonics; Optogenetics - instrumentation; Rats; Rats, Sprague-Dawley; Science; Science (multidisciplinary); Silicon - chemistry; Tomography, Optical Coherence
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/ncomms6258

Neural micro-electrode arrays that are transparent over a broad wavelength spectrum from ultraviolet to infrared could allow for simultaneous electrophysiology and optical imaging, as well as optogenetic modulation of the underlying brain tissue. The long-term biocompatibility and reliability of neural micro-electrodes also require their mechanical flexibility and compliance with soft tissues. Here we present a graphene-based, carbon-layered electrode array (CLEAR) device, which can be implanted on the brain surface in rodents for high-resolution neurophysiological recording. We characterize optical transparency of the device at >90% transmission over the ultraviolet to infrared spectrum and demonstrate its utility through optical interface experiments that use this broad spectrum transparency. These include optogenetic activation of focal cortical areas directly beneath electrodes, in vivo imaging of the cortical vasculature via fluorescence microscopy and 3D optical coherence tomography. This study demonstrates an array of interfacing abilities of the CLEAR device and its utility for neural applications. Monitoring neuronal activity in the rodent in vivo brain is commonly done using micro-electrode arrays but these devices are not normally compatible with optical technologies. Here the authors design a transparent and flexible electrode array based on graphene that allows them to combine electrophysiological recordings with optogenetic and imaging experiments.