The deubiquitinating enzyme USP11 regulates breast cancer progression by stabilizing PGAM5

• Published in: Breast cancer research : BCR Vol. 26; no. 1; pp. 135 - 13
• Main Authors: Zhang, Nannan, Wang, Quhui, Lu, Yunpeng, Wang, Feiran, He, Zhixian
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 19.09.2024; BioMed Central; BMC
• Subjects: Animals; Antibodies; Apoptosis; Apoptosis - genetics; Bioinformatics; Breast cancer; Breast Neoplasms - genetics; Breast Neoplasms - metabolism; Breast Neoplasms - pathology; Cancer; Cell cycle; Cell death; Cell growth; Cell Line, Tumor; Cell Proliferation; Development and progression; Disease Progression; Enzymes; Female; Ferroptosis; Flow cytometry; Gene Expression Regulation, Neoplastic; Glutathione; Glycolytic enzymes; Health aspects; Humans; Lipid peroxidation; Lipids; Medical prognosis; Metastases; Mice; Mitochondrial Proteins; PGAM5; Phosphoglycerate mutase; Phosphoprotein Phosphatases - genetics; Phosphoprotein Phosphatases - metabolism; Physiological aspects; Plasmids; Proteasomes; Protein Stability; Proteins; Statistical analysis; Thiolester Hydrolases - genetics; Thiolester Hydrolases - metabolism; Ubiquitin-proteasome system; Ubiquitin-specific proteinase; Ubiquitination; USP11; China; Ferroptosis; Breast cancer; PGAM5; USP11
• ISSN: 1465-542X; 1465-5411; 1465-542X
• DOI: 10.1186/s13058-024-01892-9

Breast cancer is common worldwide. Phosphoglycerate mutase 5 (PGAM5) belongs to the phosphoglycerate mutase family and plays an important role in many cancers. However, research on its role in breast cancer remains unclear. The present investigation highlights the significant expression of PGAM5 in breast cancer and its essential role in cell proliferation, invasion, apoptosis and the regulation of ferroptosis in breast cancer cells. Overexpression or knockdown of ubiquitin-specific protease 11 (USP11) promotes or inhibits the growth and metastasis of breast cancer cells, respectively, in vitro and in vivo. Mechanistically, USP11 stabilizes PGAM5 via de-ubiquitination, protecting it from proteasome-mediated degradation. In addition, the USP11/PGAM5 complex promotes breast cancer progression by activating iron death-related proteins, indicating that the synergy between USP11 and PGAM5 may serve as a predictor of disease outcome and provide a new treatment strategy for breast cancer.