Fractional volume of placental vessels in women with diabetes using a novel stereological 3D power Doppler technique

• Published in: Placenta (Eastbourne) Vol. 34; no. 11; pp. 1002 - 1008
• Main Authors: Jones, N.W., Deshpande, R., Mousa, H.A., Mansell, P., Raine-Fenning, N., Bugg, G.
• Format: Journal Article
• Language: English
• Published: Kidlington Elsevier Ltd 01.11.2013; Elsevier
• Subjects: Adult; Algorithms; Biological and medical sciences; Blood flow; Diabetes; Diabetes Mellitus, Type 1 - complications; Diabetes Mellitus, Type 1 - pathology; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - pathology; Diabetic Angiopathies - diagnostic imaging; Diabetic Angiopathies - pathology; Embryology: invertebrates and vertebrates. Teratology; Female; Fundamental and applied biological sciences. Psychology; Humans; Imaging, Three-Dimensional; Internal Medicine; Longitudinal Studies; Observer Variation; Obstetrics and Gynecology; Organ Size; Pilot Projects; Placenta; Placenta - blood supply; Placenta - diagnostic imaging; Placenta - pathology; Placental Circulation; Placentation; Power Doppler; Pregnancy; Pregnancy in Diabetics - pathology; Reproducibility of Results; Stereology; Ultrasonography, Doppler; Ultrasonography, Prenatal; Ultrasound; Stereology; Placenta; Power Doppler; Diabetes; Ultrasound; Blood flow; Endocrinopathy; Sonography; Doppler ultrasound study; Human; Vertebrata; Mammalia; Fetal membrane; Volume; Diabetes mellitus; Female; Technique
• ISSN: 0143-4004; 1532-3102; 1532-3102
• DOI: 10.1016/j.placenta.2013.08.015

In maternal diabetes the placenta is large with abnormal vascular development and increased villous volume. We used a novel stereological 3D power Doppler ultrasound technique to investigate differences in-vivo in the placental fractional volume of power Doppler signal (FrVol-PD) between women with and without diabetes. We recruited 17 pregnant women with pre-gestational diabetes and 20 controls, all with anterior placentae. Each subject had ultrasound scans (Voluson 730 Expert) every 4 weeks between 12 and 32 weeks gestation. 3D power Doppler data were acquired and the placenta manually outlined using VOCAL (4D View). Power Doppler signal within the resultant volume was counted in a 3D manner adapting the random but systematic techniques used in stereology. Subjects were of similar age, BMI and parity. From 16 weeks the mean (SD) placental FrVol-PD was higher in the non-diabetic than in the diabetic group: 16 weeks – 0.125 (0.03) versus 0.108 (0.03), 20 weeks – 0.144 (0.05) versus 0.104 (0.03), 24 weeks – 0.145 (0.05) versus 0.128 (0.03), 28 weeks – 0.159 (0.05) versus 0.133 (0.02) and 32 weeks – 0.154 (0.03) versus 0.123 (0.04). These differences were significant between control and diabetic subjects [F(1,35) = 4.737, p = 0.036] and across gestation [F(3,140) = 8.294, p < 0.001]. Using a novel stereological-based ultrasound technique we have demonstrated the reliability of this application in the placenta. This technique shows promise for non-invasive assessment of placental function: further studies are required to identify if structural changes in a diabetic placenta are accompanied by altered function, manifest as reduced perfusion demonstrable in-vivo.