In situ detection and genotyping of individual mRNA molecules

Increasing knowledge about the heterogeneity of mRNA expression within cell populations highlights the need to study transcripts at the level of single cells. We present a method for detection and genotyping of individual transcripts based on padlock probes and in situ target-primed rolling-circle a...

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Bibliographic Details
Published inNature methods Vol. 7; no. 5; pp. 395 - 397
Main Authors Larsson, Chatarina, Söderberg, Ola, Grundberg, Ida, Nilsson, Mats
Format Journal Article
LanguageEnglish
Published United States Nature Publishing Group 01.05.2010
Subjects
Actins - biosynthesis
Actins - genetics
Animals
Cell Line, Tumor
Cells, Cultured
Cellular biology
Genetic aspects
Genetic transcription
Genotype & phenotype
Heterogeneity
Humans
Identification and classification
MEDICIN
MEDICINE
Messenger RNA
Mice
Mutation
Nucleic Acid Amplification Techniques - methods
Nucleic acid probes
Point Mutation
Probes
Receptor, ErbB-2 - biosynthesis
Research methodology
Ribonucleic acid
RNA
RNA, Messenger - genetics
Transcription, Genetic
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Summary:Increasing knowledge about the heterogeneity of mRNA expression within cell populations highlights the need to study transcripts at the level of single cells. We present a method for detection and genotyping of individual transcripts based on padlock probes and in situ target-primed rolling-circle amplification. We detect a somatic point mutation, differentiate between members of a gene family and perform multiplex detection of transcripts in human and mouse cells and tissue.
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SourceType-Scholarly Journals-1
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ISSN:1548-7091
1548-7105
1548-7105
DOI:10.1038/nmeth.1448