Inclusion of seasonal variation in river system microbial communities and phototroph activity increases environmental relevance of laboratory chemical persistence tests

• Published in: The Science of the total environment Vol. 733; p. 139070
• Main Authors: Southwell, Rebecca V., Hilton, Sally L., Pearson, Jonathan M., Hand, Laurence H., Bending, Gary D.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01.09.2020; Elsevier
• Subjects: Biodegradation; community structure; environment; environmental fate; Environmental realism; fungicides; inoculum; microbial communities; mineralization; OECD regulatory tests; pesticide persistence; Phototrophs; plant protection; rivers; seasonal variation; sediments; Temporal variation; toxicity; Biodegradation; Temporal variation; Environmental realism; OECD regulatory tests; Phototrophs
• ISSN: 0048-9697; 1879-1026; 1879-1026
• DOI: 10.1016/j.scitotenv.2020.139070

Regulatory tests assess crop protection product environmental fate and toxicity before approval for commercial use. Although globally applied laboratory tests can assess biodegradation, they lack environmental complexity. Microbial communities are subject to temporal and spatial variation, but there is little consideration of these microbial dynamics in the laboratory. Here, we investigated seasonal variation in the microbial composition of water and sediment from a UK river across a two-year time course and determined its effect on the outcome of water-sediment (OECD 308) and water-only (OECD 309) biodegradation tests, using the fungicide isopyrazam. These OECD tests are performed under dark conditions, so test systems incubated under non-UV light:dark cycles were also included to determine the impact on both inoculum characteristics and biodegradation. Isopyrazam degradation was faster when incubated under non-UV light at all collection times in water-sediment microcosms, suggesting that phototrophic communities can metabolise isopyrazam throughout the year. Degradation rate varied seasonally between inoculum collection times only in microcosms incubated in the light, but isopyrazam mineralisation to 14CO2 varied seasonally under both light and dark conditions, suggesting that heterotrophic communities may also play a role in degradation. Bacterial and phototroph communities varied across time, but there was no clear link between water or sediment microbial composition and variation in degradation rate. During the test period, inoculum microbial community composition changed, particularly in non-UV light incubated microcosms. Overall, we show that regulatory test outcome is not influenced by temporal variation in microbial community structure; however, biodegradation rates from higher tier studies with improved environmental realism, e.g. through addition of non-UV light, may be more variable. These data suggest that standardised OECD tests can provide a conservative estimate of pesticide persistence end points and that additional tests including non-UV light could help bridge the gap between standard tests and field studies. [Display omitted] •Non-UV light increases isopyrazam metabolism, particularly in sediment systems.•Phototrophs and heterotrophs may both metabolise isopyrazam.•Seasonal variation in isopyrazam decline only occurred in illuminated microcosms.•Tests with non-UV light could bridge the gap between laboratory and field studies.