Modulation of BMP-2-Induced Chondrogenic Versus Osteogenic Differentiation of Human Mesenchymal Stem Cells by Cell-Specific Extracellular Matrices

• Published in: Tissue engineering. Part A Vol. 19; no. 1-2; pp. 49 - 58
• Main Authors: Kwon, Sun-Hyun, Lee, Tae-Jin, Park, Jooyeon, Hwang, Ji-Eun, Jin, Min, Jang, Hyeon-Ki, Hwang, Nathaniel S., Kim, Byung-Soo
• Format: Journal Article
• Language: English
• Published: United States Mary Ann Liebert, Inc 01.01.2013
• Subjects: Animals; Animals, Newborn; Bone Morphogenetic Protein 2 - pharmacology; Bones; Cell culture; Cell Differentiation - drug effects; Cells, Cultured; Chondrogenesis - drug effects; Chondrogenesis - physiology; Extracellular Matrix - metabolism; Gene expression; Humans; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - drug effects; Mesenchymal Stromal Cells - metabolism; Original; Original Articles; Osteogenesis - drug effects; Osteogenesis - physiology; Rabbits; Rats; Rats, Sprague-Dawley; Stem cells; Tissue engineering; Tissue Engineering - methods
• ISSN: 1937-3341; 1937-335X
• DOI: 10.1089/ten.tea.2012.0245

Bone morphogenetic protein-2 (BMP-2) is known to induce both osteogenic and chondrogenic commitment of human mesenchymal stem cells (hMSCs). However, factors influencing BMP-2-dependent chondrogenic and osteogenic differentiation have not been investigated. In this study, we demonstrated that extracellular microenvironments, in the form of cell-derived matrices, play important roles in determining the specific lineage commitment of hMSCs in the presence of BMP-2. Extracellular matrices (ECMs) derived from osteoblasts and chondrocytes were utilized to regulate cell differentiation. Osteogenic and chondrogenic differentiation of hMSCs cultured on the two different cell-derived ECMs were assessed by quantitative real-time–polymerase chain reaction, immunocytochemistry, and western blot analysis. To minimize the effects of the cell-adhesion proteins contained in serum on the ECMs, hMSCs were cultured in serum-free osteogenic or chondrogenic differentiation medium. Fibronectin-, collagen type I-, or collagen type II-coated substrates were utilized as ECM controls. The ECM specific to each cell type promoted lineage-specific commitment of hMSCs in the presence of BMP-2, that is, osteoblast- and chondrocyte-derived ECM promoted osteogenic and chondrogenic commitment, respectively. Therefore, cell-specific ECMs are capable of modulating the BMP-2-induced osteogenic and chondrogenic differentiation of hMSCs.