A Method for Tissue Engineering of Cartilage by Cell Seeding on Bioresorbable Scaffolds

This chapter describes a method for the in vitro generation of a 3‐dimensional cartilage matrix from articular chondrocytes seeded onto a bioresorbable polymeric scaffold. This particular growth system was chosen for the subject of this chapter owing to the relative simplicity of the methods require...

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Published inAnnals of the New York Academy of Sciences Vol. 875; no. 1; pp. 398 - 404
Main Authors SCHREIBER, RONDA E., DUNKELMAN, NOUSHIN S., NAUGHTON, GAIL, RATCLIFFE, ANTHONY
Format Journal Article
LanguageEnglish
Published Oxford, UK Blackwell Publishing Ltd 01.06.1999
Subjects
Animals
Biocompatible Materials
Cartilage - growth & development
Cartilage - metabolism
Cattle
Culture Techniques - methods
Horses
Rabbits
Sheep
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Summary:This chapter describes a method for the in vitro generation of a 3‐dimensional cartilage matrix from articular chondrocytes seeded onto a bioresorbable polymeric scaffold. This particular growth system was chosen for the subject of this chapter owing to the relative simplicity of the methods required and the ease with which the necessary materials can be obtained. The tissue produced using this protocol is a cellular, metabolically active hyaline‐like matrix, containing the major cartilage constituents: sulfated proteoglycan, collagen type II, and water. It serves as a useful in vitro tool for studying the influence of various mechanical and chemical factors on cartilage metabolism, as well as providing an implantable material for in vivo cartilage repair studies.
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content type line 23
ISSN:0077-8923
1749-6632
DOI:10.1111/j.1749-6632.1999.tb08522.x