Genetic Detection and Characterization of Atypical Bovine Pestiviruses in Foetal Bovine Sera Claimed to be of Australian Origin

• Published in: Transboundary and emerging diseases Vol. 60; no. 3; pp. 284 - 288
• Main Authors: Xia, H., Larska, M., Giammarioli, M., De Mia, G. M., Cardeti, G., Zhou, W., Alenius, S., Belák, S., Liu, L.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01.06.2013; Hindawi Limited
• Subjects: 5' Untranslated Regions - genetics; Animals; Annan veterinärmedicin; Australia; Bayes Theorem; Bayesian analysis; Bovine viral diarrhea virus; bovine viral diarrhoea virus 1; bovine viral diarrhoea virus 2; Bovine Virus Diarrhea-Mucosal Disease - diagnosis; Bovine Virus Diarrhea-Mucosal Disease - genetics; Cattle; Cattle Diseases - diagnosis; Cattle Diseases - genetics; Cattle Diseases - virology; contamination; Diarrhea Viruses, Bovine Viral - classification; Diarrhea Viruses, Bovine Viral - genetics; Diarrhea Viruses, Bovine Viral - isolation & purification; Fetal Blood - virology; foetal bovine serum; Genetics; Genome, Viral; Other Veterinary Science; pestivirus; Phylogeny; Real-Time Polymerase Chain Reaction; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Viral - blood; RNA, Viral - genetics; Viruses; Australia
• ISSN: 1865-1674; 1865-1682; 1865-1682
• DOI: 10.1111/j.1865-1682.2012.01341.x

Summary  Two European laboratories independently detected atypical bovine pestiviral nucleic acids in three commercial batches of foetal bovine serum (FBS) that was claimed by the producers to be of Australian origin. Additional batches of FBS were obtained directly from Australia to exclude possible contamination of the Australian FBS with that of South American origin during manufacturing/packaging in European countries. RT‐PCR amplification of partial 5′untranslated region and the complete Npro gene yielded a specific band with expected size, which was confirmed by DNA sequencing. Bayesian analysis of sequence data demonstrated a closer phylogenetic relation of the newly detected atypical bovine pestiviruses to those of South American origin, which were related to the recognized bovine pestivirus species, bovine viral diarrhoea virus. Taken together, the results indicated the presence of atypical bovine pestiviruses in the Australian FBS, and most likely in Australian Continent.