A versatile electrophoresis system for the analysis of high- and low-molecular-weight proteins

• Published in: Electrophoresis Vol. 24; no. 11; pp. 1787 - 1794
• Main Authors: Tastet, Christophe, Lescuyer, Pierre, Diemer, Hélène, Luche, Sylvie, van Dorsselaer, Alain, Rabilloud, Thierry
• Format: Journal Article
• Language: English
• Published: Weinheim WILEY-VCH Verlag 01.06.2003; WILEY‐VCH Verlag; Wiley-VCH Verlag; Wiley-VCH
• Subjects: Biochemistry, Molecular Biology; Buffers; Electrophoresis, Gel, Two-Dimensional; Electrophoresis, Gel, Two-Dimensional - methods; Electrophoresis, Polyacrylamide Gel; Genomics; HeLa Cells; High-molecular-weight proteins; Humans; Life Sciences; Low-molecular-weight proteins; Molecular Weight; Proteins; Proteins - analysis; Proteomics; Proteomics - methods; Research Support, Non-U.S. Gov't; Taurine; Two-dimensional electrophoresis; electrophoresis
• ISSN: 0173-0835; 1522-2683
• DOI: 10.1002/elps.200305400

A new, versatile, multiphasic buffer system for high‐resolution sodium dodecyl sulfate‐polyacrylamide gel electrophoresis of proteins in the relative molecular weight range of 300 000–3000 Da is described. The system, based on the theory of multiphasic zone electrophoresis, allows complete stacking and destacking of proteins in the above Mr range. The buffer system uses taurine and chloride as trailing and leading ion, respectively, and Tris, at a pH close to its pKa, as the buffering counterion. Coupled with limited variation in the acrylamide concentration, this electrophoresis system allows to tailor the resolution in the 6–200 kDa Mr range, with minimal difficulties in the post electrophoretic identification processes.