Effects of cAMP-Phosphodiesterase Isozyme Inhibitor on Cytokine Production by Lipopolysaccharide-Stimulated Human Peripheral Blood Mononuclear Cells
1. The effects of cAMP-phosphodiesterase (PDE) isozyme inhibitors on the production of tumor necrosis factor α (TNF-α), and interleukins 1β and 8 (IL-1β and IL-8) by lipopolysaccharide (LPS)-stimulated human peripheral blood mononuclear cells (PBMC) were evaluated. In addition, we investigated the e...
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Published in | General pharmacology Vol. 29; no. 4; pp. 633 - 638 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
New York, NY
Elsevier Inc
01.10.1997
Elsevier |
Subjects | |
Online Access | Get full text |
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Summary: | 1.
The effects of cAMP-phosphodiesterase (PDE) isozyme inhibitors on the production of tumor necrosis factor α (TNF-α), and interleukins 1β and 8 (IL-1β and IL-8) by lipopolysaccharide (LPS)-stimulated human peripheral blood mononuclear cells (PBMC) were evaluated. In addition, we investigated the effects of dibutyryl cAMP (dbcAMP) and β-adrenergic receptor agonist on the production of these cytokines.
2.
Type IV PDE inhibitors were more effective at inhibiting the production of TNF-α and IL-1β by LPS-stimulated PBMC than a nonselective, type III or type III/IV inhibitor. In contrast, these agents had no effect on IL-8 production.
3.
Increasing concentrations of dbcAMP progressively reduced the production of TNF-α and IL-1β but not IL-8.
4.
The addition of β-agonist increased the inhibitory effect of PDE inhibitors tested on the production of TNF-α and IL-1β.
5.
Type IV PDE inhibitors could be potent pharmacological agents for the treatment of diseases in which TNF-α and IL-1β are important etiological factors. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0306-3623 1879-0011 |
DOI: | 10.1016/S0306-3623(96)00580-0 |