The effect of folic acid administration on cardiac tissue matrix metalloproteinase activity and hepatorenal biomarkers in diabetic rats

• Published in: Canadian journal of physiology and pharmacology Vol. 97; no. 9; pp. 893 - 901
• Main Authors: Mutavdzin, Slavica, Gopcevic, Kristina, Stankovic, Sanja, Uzelac, Jovana Jakovljevic, Borovic, Milica Labudovic, Djuric, Dragan
• Format: Journal Article
• Language: English
• Published: Ottawa NRC Research Press 01.09.2019; Canadian Science Publishing NRC Research Press
• Subjects: Acids; Albumin; Alkaline phosphatase; Biological markers; Biomarkers; Complications; Creatinine; Diabetes; Diabetes mellitus; Dosage; Fibrin; Fibrinogen; Folic acid; Gelatinase A; Gelatinase B; Heart; Influence; Liver; Matrix metalloproteinase; Metabolic disorders; Metalloproteinase; Pharmacology; Phosphatase; Phosphatases; Physiological aspects; Physiology; Rodents; Streptozocin; Studies; Urea; Vitamin B; Serbia
• ISSN: 0008-4212; 1205-7541
• DOI: 10.1139/cjpp-2019-0027

Diabetes mellitus (DM) is a metabolic disorder that causes severe complications. Thus, the aims of this study were to investigate the influence of DM and folic acid treatment on liver and renal biomarkers, and heart remodeling through evaluation of cardiac matrix metalloproteinase (MMP) activity. There were 4 groups: control (physiological saline 1 mL/kg, i.p., 28 days), DM (streptozotocin [STZ] 100 mg/kg in physiological saline, i.p., 1 day), folic acid (FA; 5 mg/kg, i.p., 28 days), and DM+FA (STZ 100 mg/kg, i.p., 1 day and folic acid 5 mg/kg, i.p., 28 days). Our results demonstrated increased aminotransferase and alkaline phosphatase activity, urea and creatinine concentration, and decreased albumin and fibrinogen concentration in the DM group. MMP-2 relative activity was elevated in the DM and FA groups; MMP-9 was decreased in the DM and increased in the FA group. The folic acid treatment of diabetic rats did not change aminotransferase activity; it alleviated the increase in alkaline phosphatase and the decrease in albumin and fibrinogen concentration, and reduced MMP-2 activity; however, it increased urea and creatinine concentration. In conclusion, folic acid treatment of diabetic rats has cardio- and hepato-protective effects. However, its dosing should be carefully considered because of possible renal damage.