Enzymes revolutionize the bioproduction of value-added compounds: From enzyme discovery to special applications

• Published in: Biotechnology advances Vol. 40; pp. 107520 - 51
• Main Authors: Wiltschi, Birgit, Cernava, Tomislav, Dennig, Alexander, Galindo Casas, Meritxell, Geier, Martina, Gruber, Steffen, Haberbauer, Marianne, Heidinger, Petra, Herrero Acero, Enrique, Kratzer, Regina, Luley-Goedl, Christiane, Müller, Christina A., Pitzer, Julia, Ribitsch, Doris, Sauer, Michael, Schmölzer, Katharina, Schnitzhofer, Wolfgang, Sensen, Christoph W., Soh, Jung, Steiner, Kerstin, Winkler, Christoph K., Winkler, Margit, Wriessnegger, Tamara
• Format: Journal Article
• Language: English
• Published: England Elsevier Inc 01.05.2020; Elsevier Science Ltd
• Subjects: Biocatalysis; Bioconversion; Carbohydrates; Combinatorial DNA assembly; Design optimization; Downstream processing; Enzyme cascade; Enzyme discovery; Enzyme engineering; Enzymes; Fine chemicals; Gene model; Host strain design; Materials recovery; Organic Chemicals; Process design; Synthetic Biology; Whole-cell biotransformation; Cas3; HNL; IPP; Whole-cell biotransformation; OTU; GLuc; Host strain design; DBTL cycle; FSA; rTCA cycle; SLIC; UPO; CDW; yGG; DOE; ssDNA; BCA; RBS; MALDI-TOF; SLiCE; PLA2; COMPACTER; mVOC; SEC; RE; DMAPP; ω-TA; CalB; FACS; FDH; PHB; NTR; bp; TPL; NADH; NADP; oePCR; Downstream processing; LIC; FMO; 3PG; M-PERL; TA; βGal; MoClo; VEGAS; CLIVA; DC/4-HB cycle; PAL; AldDH; ATP; Process design; PAT; CPO; CPR; ADH; SHA; ISPR; LC-MS/MS; NDP-sugars; eYAC; PBM; CYP; AEC; DSP; ee; UDP; Combinatorial DNA assembly; Enzyme cascade; ncAA; hFMOs; DKR; HMMs; IRED; MEC; DHAP; CAP; CAR; CRC; CAT; ROS; PKS; AADH; LCR; MES; AlaDH; Gene model; dsDNA; PCR; SAM; NADPH; PTE; cAA; EFC; SRA; PLA; Enzyme engineering; FL; nt; MFC; PLICing; TTL; CBM; CBB cycle; CPEC; 7-ACA; Pls; RuBP; CLEA; Biocatalysis; NRPS; BAC; DMP; BVMO; GST; RuBisCO; T4L; MODAL; NAD; 3-HP/4-HB cycle; SCG; 3-HP pathway; kbp; API; Enzyme discovery; PET; ABPP
• ISSN: 0734-9750; 1873-1899
• DOI: 10.1016/j.biotechadv.2020.107520

Competitive sustainable production in industry demands new and better biocatalysts, optimized bioprocesses and cost-effective product recovery. Our review sheds light on the progress made for the individual steps towards these goals, starting with the discovery of new enzymes and their corresponding genes. The enzymes are subsequently engineered to improve their performance, combined in reaction cascades to expand the reaction scope and integrated in whole cells to provide an optimal environment for the bioconversion. Strain engineering using synthetic biology methods tunes the host for production, reaction design optimizes the reaction conditions and downstream processing ensures the efficient recovery of commercially viable products. Selected examples illustrate how modified enzymes can revolutionize future-oriented applications ranging from the bioproduction of bulk-, specialty- and fine chemicals, active pharmaceutical ingredients and carbohydrates, over the conversion of the greenhouse-gas CO2 into valuable products and biocontrol in agriculture, to recycling of synthetic polymers and recovery of precious metals.