Effect of calcium citrate on bone integration in a rabbit femur defect model

• Published in: Asian Pacific journal of tropical medicine Vol. 5; no. 4; pp. 310 - 314
• Main Authors: Zhang, Wei, Wang, Wei, Chen, Qing-Yu, Lin, Zhong-Qin, Cheng, Shao-Wen, Kou, Dong-Quan, Ying, Xiao-Zhou, Shen, Yue, Cheng, Xiao-Jie, Nie, Peng-Fei, Li, Xiu-Cui, Rompis, Ferdinand An, Huang, Hang, Zhang, Hua, Mu, Zhong-Lin, Peng, Lei
• Format: Journal Article
• Language: English
• Published: India Elsevier B.V 01.04.2012; Department of 0rthopaedic Surgery, the Second Affiliated Hospital of Wenzhou Medical College,109 Xue Yuan Xi Road, Wenzhou 325000, China%Department of 0rthopaedic Surgery, Hospital of Integrated Traditional Chinese and Western Medicine of Wenzhou, Wenzhou, China%Department of Respiratory Medicine, Yuying Children's Hospital Affiliated to Wenzhou Medical College, Wenzhou, Zhejiang, China%Trauma Center of the Affiliated Hospital of Hainan Medical College, 31 Long Hua Road, Haikou, China%Haikou120 Emergency Center, 43 Renmin Da Dao, Haikou 570208, Hainan Province, China%International Nursing School of Hainan Medical College, 3 Xueyuan Road, Haikou 571199, Hainan Province, China%Graduate Department of Hainan Medical College, 3 Xueyuan Road, Haikou 571199, Hainan Province, China%The Second Affiliated Hospital of Wenzhou Medical College, Trauma Center of the Affiliated Hospital of Hainan Medical College, 109 Xue Yuan Xi Road, Wenzhou 325000, China
• Subjects: Animals; Bone; Bone Density Conservation Agents - pharmacology; Bone Regeneration - drug effects; Calcium; Calcium citrate; Calcium Citrate - pharmacology; citrate; Experiment; Femur - diagnostic imaging; Femur - drug effects; Fracture; Fracture healing; healing; Male; Rabbits; Radiography; Random Allocation; Wound Healing - drug effects; Japan; INW, Japan; Fracture healing; Bone; Experiment; Calcium citrate
• ISSN: 1995-7645; 2352-4146; 2352-4146
• DOI: 10.1016/S1995-7645(12)60045-5

<正>Objective:To explore effect of calcium citrate on bone integration in a rabbit femur defect model, and to compare the bone formation with different sizes by radiological and histological study. Methods:Twenty-four male Japanese white rabbits were randomly divided into three groups (Group A.B.C)in this study.Under anesthesia,defects of four sizes(1.2,1.5,2.0 and 2.5 mm) were created in each of the rabbits.Commercially pure calcium citrate powder was placed inside the medullary compartment of the femur(Experimental),while in the contralateral femur(Control) nothing was implanted.The defects were analyzed using radiography and histological analysis by using Imagepro-Plus 6.0 software after animal was sacrificed at 4th(Group A),6th(Group B) and 8th(Group C) weeks postoperatively.Four samples were analyzed for each size of defect and each healing period.Results:The histological and the radiologic evaluation were performed after sacrification of all rabbits on postoperative 4th and 6th weeks,It showed significant difference between the experimental group and the control group when these defects were less than or equal to 2.0 mm.No statistical difference was observed when these defects were larger than 2.0 mm at all healing periods except at the 4th week.Conclusions:Calcium citrate affects the early periods of hone defects healing mechanism in Japanese white rabbits positively,especially when the defect is not too large,We suggest further studies on calcium citrate to determine the effects of various dosages,administration ways and the experimental time on the bone defects.