Anti-inflammatory effect of prednisolone on the growth of human liver fluke in experimental opisthorchiasis

• Published in: Parasitology research (1987) Vol. 110; no. 6; pp. 2271 - 2279
• Main Authors: Juasook, Amornrat, Boonmars, Thidarut, Kaewkes, Sasithorn, Loilome, Watcharin, Veteewuthacharn, Kulathida, Wu, Zhiliang, Yongvanit, Puangrat
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01.06.2012; Springer
• Subjects: Animals; Anti-Inflammatory Agents - administration & dosage; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Cricetinae; Cyclooxygenase 2 - analysis; Development and progression; Disease Models, Animal; Fluke infections; Fundamental and applied biological sciences. Psychology; General aspects; General aspects and techniques. Study of several systematic groups. Models; Health aspects; Histocytochemistry; Humans; Immunohistochemistry; Immunology; Invertebrates; Male; Medical Microbiology; Mesocricetus; Microbiology; Microscopy; Opisthorchiasis - drug therapy; Opisthorchiasis - parasitology; Opisthorchiasis - pathology; Opisthorchis - drug effects; Opisthorchis - growth & development; Original Paper; Parasite Load; Physiological aspects; Prednisolone; Prednisolone - administration & dosage; Treatment Outcome; Thailand; Japan; Syrian Hamster; Intrahepatic Bile Duct; Primary Sclerosing Cholangitis; Ventral Sucker; Prednisolone; Human; Parasite; Experimental study; Growth; Liver
• ISSN: 0932-0113; 1432-1955
• DOI: 10.1007/s00436-011-2759-8

Opisthorchis viverrini is one of the risk factors for cholangiocarcinoma (CCA) development and is endemic in Southeast Asia including Thailand. CCA is induced by chronic inflammation from a combination of mechanical damage, parasite secretions, and immunopathology. Chronic infection with O. viverrini has been associated with several hepatobiliary diseases which affect the development of hepatobiliary cancer and CCA. Therefore, reducing the pathogenesis from O. viverrini infection may be one of the choices to reduce the risk of cholangiocarcinoma development. Prednisolone is one of the steroidal anti-inflammatory drugs that suppress inflammation, and its use has risen continuously in recent years. We therefore investigated the effect of prednisolone on pathological changes in Syrian hamster opisthorchiasis, in terms of gross and histopathological changes, worm size, eggs per gram, eggs per worm, and immunohistochemical staining for COX2. Syrian hamsters were divided into three groups: uninfected control; O. viverrini -infected (OV); and O. viverrini -infected plus prednisolone administration (OVP). The results showed an anti-inflammatory effect in the OVP group by a reduction of the inflammatory cells surrounding the intrahepatic bile ducts. However, in addition, parasite sizes for all times of observation were larger than for other groups, which was also correlated with increased eggs per worm and eggs per gram of feces. This result suggests that prednisolone is useful in suppressing inflammation in Syrian hamster opisthorchiasis, whereas it was also beneficial for parasites by enhancing their reproductive development. To clarify the mechanism of this phenomenon, further studies are under investigation.