Evaluation of in vitro antioxidant and apoptotic activities of Cyperus rotundus
Objective:To evaluate in vitro antioxidant and apoptotic activities of Crperus rotundas(C.rotundus).Methods:The phytochemical study and the antioxidant activities of both methanol and aqueous extracts from C.rotundus aerial part were determined.In addition,these extracts were also investigated for t...
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Published in | Asian Pacific journal of tropical medicine Vol. 7; no. 2; pp. 105 - 112 |
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Main Authors | , , , , , , |
Format | Journal Article |
Language | English |
Published |
India
Elsevier B.V
01.02.2014
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Subjects | |
Online Access | Get full text |
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Summary: | Objective:To evaluate in vitro antioxidant and apoptotic activities of Crperus rotundas(C.rotundus).Methods:The phytochemical study and the antioxidant activities of both methanol and aqueous extracts from C.rotundus aerial part were determined.In addition,these extracts were also investigated for their cytotoxic and apoptotic activities.The major compound of the methanol extract was isolated.Both metlianol and aqueous extracts(300,150,and 50μg/mL)were evaluated for their antioxidant activity by the xanthine/xanthine oxidase assay system.However,16,8,and 4 mg/mL of each extract were tested to investigate their OH.formation scavenging potential.Aqueous extract(800,400.and 200μg/mL)and melhunol extract(350,175,and 88μg/mL)were tested against lipid peroxidation,induced by 75μM H2O2,The cytotoxicity(by MTT assay)and cell DNA fragmentation of both extracts were evaluated Inwards K562 and L1210 cell lines.The major compound was obtained from the butanol fraction of methanol extract and its structure was determined by KMN spectroscopic analysis.Results:The methanol and aqueous extracts showed respectively,88%and 19%inhibition of xanthine oxidase activity.Vet.the same extracts inhibited lipid peroxidation by 61.5%and 42.0%.respectively.Roth extracts inhibited OH.formation by 27.1%and 25.3%,respectively.Only methanol cxtract induced DNA degradation.Orientin was determined as the major compound isolated from the butanol fraction of metlianol extract.Conclusions:It appears that C.rotundus extracts exhibit a potential use as a natural antioxidant and an apoptosis inducer. |
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Bibliography: | Kilani-Jaziri Soumaya;Ghedira Zied;Nasr Nouha;Krifa Mounira;Ghedira Kamel;Franca Dijoux Marie Genviève;Ghedira Chekir Leila;Unité de Substances Naturelles Bioactives et Biotechnologies UR12ES12.Universite de Monastic,Faculte de Pharmacie de Monastir;Laboratoire de Biologie Moleculaire et Cellulaire,Faculté de Medecine Dentaire de Monastir,Universite de Monastir,Rue Aricenne,5019 Monastir,Tunisie;Laboratoire de Botanique.Pharmacognosie et Phytothérapie,UMR CNRS 5557 Ecologie Microbienne;Institut des Sciences Pharmaceutiques et Biologiques-Faculte de Pharmacie Université Claude Bernard,Btiment Netien-8 Avenue Rockefeller-69373 Lyon cedex 08,France ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 1995-7645 2352-4146 |
DOI: | 10.1016/S1995-7645(14)60004-3 |