Effects of simulated nitrogen deposition on the soil microbial community diversity of a Deyeuxia angustifolia wetland in the Sanjiang Plain, Northeastern China

• Published in: Annals of microbiology Vol. 72; no. 1; pp. 1 - 13
• Main Authors: Zhang, Rong-Tao, Liu, Ying-Nan, Zhong, Hai-Xiu, Chen, Xiang-Wei, Sui, Xin
• Format: Journal Article
• Language: English
• Published: Heidelberg Springer 01.12.2022; Springer Nature B.V; BMC
• Subjects: Acidic soils; Acidification; Analysis; Bacteria; Biological diversity; Environmental aspects; Microbiomes; Miseq sequence; Next-generation sequencing; Nitrogen; Relative abundance; rRNA 16S; Sanjiang plain; Soil microbiology; Soil microorganisms; Soil physico-chemistry properties; Structure composition; Structure-function relationships; Terrestrial ecosystems; Wetlands; China
• ISSN: 1590-4261; 1869-2044
• DOI: 10.1186/s13213-022-01666-8

Abstract Purpose The soil microbial community is an important bioactive component of terrestrial ecosystems. Its structural and functional diversity directly affects carbon and nitrogen processes. This study aimed to investigate the variations in the diversity and composition of soil bacterial communities in a wetland with different nitrogen deposition conditions. Methods A long-term simulated nitrogen deposition experiment was conducted in the Ecological Locating Research Station of the Institute of Nature and Ecology of Heilongjiang Academy of Sciences. Three different treatments were evaluated, including low nitrogen (LK; 40 kg N·hm -2 ·a -1 ), high nitrogen (HN; 80 kg N·hm -2 ·a -1 ), and control (CK; 0 kg N·hm -2 ·a -1 ). Bacterial 16S rDNA was then sequenced and analyzed using the next-generation sequencing technology. Result Higher levels of N deposition resulted in an α-diversity increase followed by a decrease, with significant reductions in the HN treatment. Simulated nitrogen deposition resulted in changes in the structure and abundance of bacterial communities in wetland soils. The dominant phyla in all three plots were Proteobacteria and Acidobacteria. Compared with CK, the relative abundance of Chloroflexi increased significantly under the HN treatment ( P < 0.05), whereas the relative abundance of Firmicutes and Bacteroidetes decreased significantly ( P < 0.05). Nitrogen input changed the composition and relative abundance of the bacterial community, which was possibly due to N-induced soil acidification. Conclusion This study thus provides a theoretical basis for predicting the effects of atmospheric nitrogen deposition on soil microorganisms, as well as changes in the wetland ecosystem in Sanjiang Plain.