Universal Marker and Detection Tool for Human Sarcoma Circulating Tumor Cells

• Published in: Cancer research (Chicago, Ill.) Vol. 74; no. 6; pp. 1645 - 1650
• Main Authors: Satelli, Arun, Mitra, Abhisek, Cutrera, Jeffry J., Devarie, Marcos, Xia, Xueqing, Ingram, Davis R., Dibra, Denada, Somaiah, Neeta, Torres, Keila E., Ravi, Vinod, Ludwig, Joseph A., Kleinerman, Eugenie S., Li, Shulin
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 15.03.2014
• Subjects: Antibodies, Monoclonal - chemistry; Antineoplastic agents; Base Sequence; Biological and medical sciences; Biomarkers, Tumor - metabolism; Cell Line, Tumor; DNA Mutational Analysis; Flow Cytometry; Fluorescent Antibody Technique, Indirect; Gene Amplification; Human Umbilical Vein Endothelial Cells - metabolism; Humans; Medical sciences; Microscopy, Fluorescence; Multiple tumors. Solid tumors. Tumors in childhood (general aspects); Neoplastic Cells, Circulating - metabolism; Pharmacology. Drug treatments; Prognosis; Sarcoma - diagnosis; Sarcoma - metabolism; Sarcoma - pathology; Sensitivity and Specificity; Single-Cell Analysis; Tumor Suppressor Protein p53 - genetics; Tumors; Vimentin - immunology; Vimentin - metabolism; Human; Sarcoma; Biological marker; Malignant tumor; Diagnosis; Detection; Tumor cell; Cancer
• ISSN: 0008-5472; 1538-7445; 1538-7445
• DOI: 10.1158/0008-5472.CAN-13-1739

To date, no specific marker exists for the detection of circulating tumor cells (CTC) from different types of sarcomas, though tools are available for detection of CTCs in peripheral blood of patients with cancer for epithelial cancers. Here, we report cell-surface vimentin (CSV) as an exclusive marker on sarcoma CTC regardless of the tissue origin of the sarcoma as detected by a novel monoclonal antibody. Utilizing CSV as a probe, we isolated and enumerated sarcoma CTCs with high sensitivity and specificity from the blood of patients bearing different types of sarcoma, validating their phenotype by single cell genomic amplification, mutation detection, and FISH. Our results establish the first universal and specific CTC marker described for enumerating CTCs from different types of sarcoma, thereby providing a key prognosis tool to monitor cancer metastasis and relapse. Cancer Res; 74(6); 1645–50. ©2014 AACR.