Novel mechanism by which extracellular vesicles derived from Lactobacillus murinus alleviates deoxynivalenol-induced intestinal barrier disruption

• Published in: Environment international Vol. 185; p. 108525
• Main Authors: Fan, Jinping, Zhang, Yuhan, Zuo, Minyu, Ding, Shixuan, Li, Jingjing, Feng, Shengkai, Xiao, Yingping, Tao, Shiyu
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01.03.2024; Elsevier
• Subjects: Deoxynivalenol; environment; Extracellular vesicles; Gut barrier; interleukin-10; intestinal microorganisms; intestines; Lactobacillus murinus; Macrophage; macrophages; phenotype; pollutants; probiotics; protein synthesis; risk; tight junctions; toxicity; LmEVs; D-LA; Extracellular vesicles; TNF; iNOS; DON; ACE; CON; EVs; IL; Arg1; TBST; L. murinus; Deoxynivalenol; FMT; TGF; DAO; ZO-1; Gut barrier; CD206; NTA; Lactobacillus murinus; TEM; Macrophage; ELISA; TLR2
• ISSN: 0160-4120; 1873-6750; 1873-6750
• DOI: 10.1016/j.envint.2024.108525

[Display omitted] •DON-induced gut toxicity is partly mediated by gut microbiota.•L. murinus is a potential probiotic against DON.•L. murinus and its EVs effectively alleviated DON-induced intestinal barrier disorder.•LmEVs upregulate TLR2 to promote M2 macrophage polarization to protect the gut barrier. Deoxynivalenol (DON) is a common environmental pollutant that poses a serious health risk to humans worldwide. This study was aim to explore whether gut microbiota is involved in DON-induced intestinal toxicity as well as to reveal effect of probiotics derived from gut microbiota in protecting intestinal barrier and to elucidate mechanism. We found that DON caused disturbed gut microbiota, particularly Lactobacillus murinus (L. murinus) deficiency. DON enhanced M1 macrophage polarization and decreased tight junction protein expression. Microbiota transplantation experiments showed that transfer of DON-disrupted microbiota to healthy mice resulted in delivery of DON-induced intestinal toxicity. Besides, DON lost its damaging effect on macrophage and intestinal barrier in antibiotic-treated mice. Further intervention experiments revealed that L. murinus induce macrophage conversion from M1 to M2 phenotype through secreted extracellular vesicles (EVs) to alleviate DON-induced intestinal barrier disruption. Mechanistically, EVs activate TLR2 to promote M2 macrophage polarization and release IL-10, which in turn enhances intestinal barrier function. Upon successful translation of its efficacy into clinical practice, EVs created from L. murinus could be a novel possible treatment strategy for DON-induced gut disease.