High resolution size analysis of ADP-ribose polymers using modified DNA sequencing gels

• Published in: Nucleic acids research Vol. 18; no. 8; p. 2194
• Main Authors: PANZETER, P. L, ALTHAUS, F. R
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 25.04.1990
• Subjects: Adenosine Diphosphate Ribose - analysis; Biological and medical sciences; Electrophoresis; Fundamental and applied biological sciences. Psychology; Methods; Miscellaneous; Molecular and cellular biology; Molecular genetics; Polymers - analysis; Posttranslational modification; Autoradiography; Electrophoresis; ADP ribosylation; Method; DNA binding protein

Mammalian cells respond to DNA damage by producing polymers of ADP-ribose (ADPR) on certain DNA-binding proteins. Synthesis of variably-sized ADPR polymers correlates with modified protein function. Here we present a high resolution electrophoresis technique for separating and quantifying picomole q...