High resolution size analysis of ADP-ribose polymers using modified DNA sequencing gels

Mammalian cells respond to DNA damage by producing polymers of ADP-ribose (ADPR) on certain DNA-binding proteins. Synthesis of variably-sized ADPR polymers correlates with modified protein function. Here we present a high resolution electrophoresis technique for separating and quantifying picomole q...

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Bibliographic Details
Published inNucleic acids research Vol. 18; no. 8; p. 2194
Main Authors PANZETER, P. L, ALTHAUS, F. R
Format Journal Article
LanguageEnglish
Published Oxford Oxford University Press 25.04.1990
Subjects
Adenosine Diphosphate Ribose - analysis
Biological and medical sciences
Electrophoresis
Fundamental and applied biological sciences. Psychology
Methods
Miscellaneous
Molecular and cellular biology
Molecular genetics
Polymers - analysis
Posttranslational modification
Autoradiography
Electrophoresis
ADP ribosylation
Method
DNA binding protein
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Summary:Mammalian cells respond to DNA damage by producing polymers of ADP-ribose (ADPR) on certain DNA-binding proteins. Synthesis of variably-sized ADPR polymers correlates with modified protein function. Here we present a high resolution electrophoresis technique for separating and quantifying picomole quantities of polymers up to 60 residues long. This technique allows one to study the role of specific polymer size patterns in the regulation of chromatin protein function.
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ISSN:0305-1048
1362-4962
DOI:10.1093/nar/18.8.2194