Metacyclic form-specific variable surface glycoprotein-encoding genes of Trypanosoma (Nannomonas)congolense

• Published in: Gene Vol. 113; no. 2; pp. 139 - 148
• Main Authors: Eshita, Yuki, Urakawa, Toyohiko, Hirumi, Hiroyuki, Fish, Wallace R., Majiwa, Phelix A.O.
• Format: Journal Article
• Language: English
• Published: Lausanne Elsevier B.V 15.04.1992; Amsterdam Elsevier; New York, NY
• Subjects: Amino Acid Sequence; amino acid sequences; Animals; antibodies; antibody screening; Antigens, Protozoan - genetics; Base Sequence; Biological and medical sciences; Blotting, Northern; Blotting, Western; cDNA; developmental stages; DNA - genetics; Electrophoresis, Polyacrylamide Gel; epitopes; Fundamental and applied biological sciences. Psychology; genbank/m4802 [sic]; genbank/m4803 [sic]; genes; Genes. Genome; messenger RNA; metacyclic antigen-encoding gene; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; nucleotide sequence; nucleotide sequences; phage λ library; prediction; Recombinant DNA; RNA, Messenger - genetics; Sequence Homology, Nucleic Acid; surface antigens; transcription (genetics); Transcription, Genetic; Trypanosoma congolense; Trypanosoma congolense - genetics; tsetse fly; variant surface glycoprotein; variant surface glycoproteins; Variant Surface Glycoproteins, Trypanosoma - genetics; ORF; nt; VAT; phage λ library; Recombinant DNA; bp; m-form; bs; ImGS; kb; tsetse fly; aa; YNat; PSG; antibody screening; PAGE; VSG; m; βGal; mVSG; CRD; ILNaR; IPTG; DEAE; metacyclic antigen-encoding gene; pfu; Protozoa; Life cycle; Type specificity; Complementary DNA; Antigenic determinant; Gene; Nucleotide sequence; Sequence alignment; Trypanosoma congolense; Rhizoflagellata; Glycoproteins
• ISSN: 0378-1119; 1879-0038
• DOI: 10.1016/0378-1119(92)90389-7

A complementary DNA expression library in phage λgt11 was synthesized using MRNA from in vitro-produced metacyclic forms of a clone of Trypanosoma (Nannomonas) congolense. The unamplified library was screened with antiserum from a goat immune to infection with metacyclic (m)-forms of T. congolense ILRAD Nannomonas antigen repertoire 2(ILNaR2). Of the 100 antiserum-reactive phage clones identified, 22 were analyzed further: 21 of the clones contained overlapping portions of a single transcript, while one other contained a different transcript. Northern blot analyses indicated that the sequences contained in the clones were transcribed only by m-forms of ILNaR2. Immunological and sequence analyses indicated that the two different cloned sequences encode m-form-specific variable surface glycoproteins.